Invitrogen

HMGCR Polyclonal Antibody

HMGCR Antibody in Immunocytochemistry (ICC/IF)

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HMGCR Antibody (PA5-37367) in ICC/IF

Immunofluorescence analysis of HMGCR was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with HMGCR Polyclonal Antibody (Product # PA5-37367) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More

HMGCR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCR Antibody in Immunohistochemistry (IHC)

HMGCR Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

HMGCR Antibody — Fig. 6 a , b , Representative images and quantification of migrated or invaded LNCaP cells in the migration and invasion assay. LNCaP cells were transfected with the indicated control or target siRNAs against PML or/and SREBP-1 ( a ) or were pretreated with DMSO, 10 mug/ml TOFA or 10 muM simvastatin ( b ) for 48 h, then subjected to migration (24 h) or invasion (48 h) assays. Scale bars, 50 mum. c - g , Gross anatomy of representative Pten pc-/- ; Pml pc-/- tumors ( c ), quantification of tumor weight ( d ) and the incidence of metastasis ( e ) and immunoblot ( f ) and IHC ( g ) analyses of tumors from Pten pc-/- ; Pml pc-/- mice after treatment with vehicle or 15 mg/kg fatostatin for two months. Scale bars, 5 mm ( c ) and 50 mum ( g ). Arrowheads in g indicate apoptotic cells. In a and b , the results of one representative experiment are shown ( n = 5 experiments). Data are from three independent cultures (4 fields per insert). In d , n = 6 mice per treatment. Data shown in a , b and d are mean +- s.e.m. Student's t test (two tailed) was used to determine significance. Uncropped images for f are shown in Supplementary Fig. 7 . Numbers to left of blot indicate molecular weight (kDa). {TM}

产品信息

PA5-37367

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:4,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50-1:100

免疫细胞化学 (ICC/IF)

1:50-1:200

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant full length human HMGCR

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2554032

产品详细信息

This antibody detects endogenous protein at a molecular weight of 97 kDa.

Purity is >95% by SDS-PAGE.

靶标信息

3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR) is a key enzyme in the mevalonate pathway, which is critical for the biosynthesis of cholesterol and other isoprenoids. HMGCR catalyzes the conversion of HMG-CoA to mevalonate, a rate-limiting step in the production of cholesterol. Due to its central role in cholesterol synthesis, HMGCR is a primary target for statin drugs, which are used to lower cholesterol levels and reduce the risk of cardiovascular disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Identification of the novel role of sterol regulatory element binding proteins (SREBPs) in mechanotransduction and intraocular pressure regulation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wang T,Soundararajan A,Rabinowitz J,Jaiswal A,Osborne T,Pattabiraman PP

Published figure using HMGCR polyclonal antibody (Product # PA5-37367) in Western Blot

Wed Nov 01 00:00:00 UTC 2023

Dyrk1b promotes hepatic lipogenesis by bypassing canonical insulin signaling and directly activating mTORC2 in mice.

The Journal of clinical investigation

Bhat N,Narayanan A,Fathzadeh M,Kahn M,Zhang D,Goedeke L,Neogi A,Cardone RL,Kibbey RG,Fernandez-Hernando C,Ginsberg HN,Jain D,Shulman GI,Mani A

PA5-37367 was used in Western Blot to investigate DYRK1B levels in the liver of patients with nonalcoholic steatohepatitis (NASH)and in mice fed with a high-fat, high-sucrose diet.

Tue Feb 01 00:00:00 UTC 2022

Dyrk1b promotes hepatic lipogenesis by bypassing canonical insulin signaling and directly activating mTORC2 in mice.

The Journal of clinical investigation

Bhat N,Narayanan A,Fathzadeh M,Kahn M,Zhang D,Goedeke L,Neogi A,Cardone RL,Kibbey RG,Fernandez-Hernando C,Ginsberg HN,Jain D,Shulman GI,Mani A

PA5-37367 was used in Western Blot to investigate DYRK1B levels in the liver of patients with nonalcoholic steatohepatitis (NASH)and in mice fed with a high-fat, high-sucrose diet.

Tue Feb 01 00:00:00 UTC 2022

Limb-girdle muscular dystrophy type 2B causes HDL-C abnormalities in patients and statin-resistant muscle wasting in dysferlin-deficient mice.

Skeletal muscle

White Z,Sun Z,Sauge E,Cox D,Donen G,Pechkovsky D,Straub V,Francis GA,Bernatchez P

Published figure using HMGCR polyclonal antibody (Product # PA5-37367) in Western Blot

Tue Nov 29 00:00:00 UTC 2022

Cholesterol biosynthesis defines oligodendrocyte precursor heterogeneity between brain and spinal cord.

Cell reports

Khandker L,Jeffries MA,Chang YJ,Mather ML,Evangelou AV,Bourne JN,Tafreshi AK,Ornelas IM,Bozdagi-Gunal O,Macklin WB,Wood TL

PA5-37367 was used in Western Blot to examine cholesterol regulation in oligodendrocyte differentiation.

Tue Mar 01 00:00:00 UTC 2022

Cholesterol biosynthesis defines oligodendrocyte precursor heterogeneity between brain and spinal cord.

Cell reports

Khandker L,Jeffries MA,Chang YJ,Mather ML,Evangelou AV,Bourne JN,Tafreshi AK,Ornelas IM,Bozdagi-Gunal O,Macklin WB,Wood TL

PA5-37367 was used in Western Blot to examine cholesterol regulation in oligodendrocyte differentiation.

Tue Mar 01 00:00:00 UTC 2022

TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.

The Journal of cell biology

Ho WY,Chang JC,Lim K,Cazenave-Gassiot A,Nguyen AT,Foo JC,Muralidharan S,Viera-Ortiz A,Ong SJM,Hor JH,Agrawal I,Hoon S,Arogundade OA,Rodriguez MJ,Lim SM,Kim SH,Ravits J,Ng SY,Wenk MR,Lee EB,Tucker-Kellogg G,Ling SC

Published figure using HMGCR polyclonal antibody (Product # PA5-37367) in Immunocytochemistry

Mon Sep 06 00:00:00 UTC 2021

A Retrospective Hospital-Based Study of HMGCR Expression in HER2 IHC 2+ and 3+ Breast Cancer.

Asian Pacific journal of cancer prevention : APJCP

Mohamed M,Mohd Nafi SN,Jaafar H,Paiman NM

Published figure using HMGCR polyclonal antibody (Product # PA5-37367) in Immunohistochemistry

Thu Jul 01 00:00:00 UTC 2021

GPR146 Deficiency Protects against Hypercholesterolemia and Atherosclerosis.

Cell

Yu H,Rimbert A,Palmer AE,Toyohara T,Xia Y,Xia F,Ferreira LMR,Chen Z,Chen T,Loaiza N,Horwitz NB,Kacergis MC,Zhao L,Soukas AA,Kuivenhoven JA,Kathiresan S,Cowan CA

PA5-37367 was used in Western Blotting to demonstrate that orphan G protein-coupled receptor 146 (GPR146) promotes activity of hepatic sterol regulatory element binding protein 2 (SREBP2) through activation of the extracellular signal-regulated kinase (ERK) signaling pathway, thereby regulating hepatic very low-density lipoprotein (VLDL) secretion, and subsequently circulating low-density lipoprotein cholesterol (LDL-C) and triglycerides (TG) levels.

Wed Nov 27 00:00:00 UTC 2019

Eicosapentaenoic acid in combination with EPHA2 inhibition shows efficacy in preclinical models of triple-negative breast cancer by disrupting cellular cholesterol efflux.

Oncogene

Torres-Adorno AM,Vitrac H,Qi Y,Tan L,Levental KR,Fan YY,Yang P,Chapkin RS,Eckhardt BL,Ueno NT

Published figure using HMGCR polyclonal antibody (Product # PA5-37367) in Western Blot

Fri Mar 01 00:00:00 UTC 2019

Lipid and glucose metabolism in hepatocyte cell lines and primary mouse hepatocytes: a comprehensive resource for in vitro studies of hepatic metabolism.

American journal of physiology. Endocrinology and metabolism

Nagarajan SR,Paul-Heng M,Krycer JR,Fazakerley DJ,Sharland AF,Hoy AJ

PA5-37367 was used in Western Blotting to describe substrate metabolism of cultured AML12, IHH, and PH5CH8 cells, hepatocellular carcinoma-derived HepG2s, and primary mouse hepatocytes.

Mon Apr 01 00:00:00 UTC 2019

An aberrant SREBP-dependent lipogenic program promotes metastatic prostate cancer.

Nature genetics

Chen M,Zhang J,Sampieri K,Clohessy JG,Mendez L,Gonzalez-Billalabeitia E,Liu XS,Lee YR,Fung J,Katon JM,Menon AV,Webster KA,Ng C,Palumbieri MD,Diolombi MS,Breitkopf SB,Teruya-Feldstein J,Signoretti S,Bronson RT,Asara JM,Castillo-Martin M,Cordon-Cardo C,Pandolfi PP

Published figure using HMGCR polyclonal antibody (Product # PA5-37367) in Immunohistochemistry

Thu Feb 01 00:00:00 UTC 2018

An aberrant SREBP-dependent lipogenic program promotes metastatic prostate cancer.

Nature genetics

Published figure using HMGCR polyclonal antibody (Product # PA5-37367) in Immunohistochemistry

Thu Feb 01 00:00:00 UTC 2018

Concomitant attenuation of HMG-CoA reductase expression potentiates the cancer cell growth-inhibitory effect of statins and expands their efficacy in tumor cells with epithelial characteristics.

Oncotarget

Ishikawa T,Hosaka YZ,Beckwitt C,Wells A,Oltvai ZN,Warita K

PA5-37367 was used in Western Blotting to show that attenuation of HMGCR expression in the presence of atorvastatin leads to stronger growth inhibition than dual target blockade of the mevalonate pathway in relatively statin resistant cell lines.

Fri Jun 29 00:00:00 UTC 2018

生物信息学

蛋白别名: 3-hydroxy-3-methylglutaryl CoA reductase (NADPH); 3-hydroxy-3-methylglutaryl-CoA reductase; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMDH; HMG CoA reductase; HMG CoAR; HMG-CoA reductase; hydroxymethylglutaryl-CoA reductase; MGC103269

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基因别名: 3H3M; HMG-CoAR; HMGCR; LDLCQ3; Red

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UniProt ID: (Human) P04035, (Mouse) Q01237, (Rat) P51639

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Entrez Gene ID: (Human) 3156, (Mouse) 15357, (Rat) 25675

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功能