Invitrogen

HMGCS1 Polyclonal Antibody

HMGCS1 Antibody in Immunocytochemistry (ICC/IF)

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HMGCS1 Antibody (PA5-29488) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of HMGCS1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGCS1 Polyclonal Antibody (Product # PA5-29488) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

HMGCS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HMGCS1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

HMGCS1 Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-29488

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

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免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human, Mouse

已发表种属

Horse, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 194 and 455 of Human HMGCS1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.39 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2546964

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse liver.

Predicted reactivity: Mouse (97%), Rat (97%), Pig (95%), Chicken (82%), Rhesus Monkey (99%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration.

Nature communications

Molina-Gonzalez I,Holloway RK,Jiwaji Z,Dando O,Kent SA,Emelianova K,Lloyd AF,Forbes LH,Mahmood A,Skripuletz T,Gudi V,Febery JA,Johnson JA,Fowler JH,Kuhlmann T,Williams A,Chandran S,Stangel M,Howden AJM,Hardingham GE,Miron VE

PA5-29488 was used in Immunohistochemistry-immunofluorescence to identify that astrocyte-oligodendrocyte interaction regulates remyelination, and reveal a drug strategy for central nervous system regeneration centred on targeting this interaction.

Thu Jun 08 00:00:00 UTC 2023

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

PA5-29488 was used in Western Blot to identify the thrombin receptor (Protease Activated Receptor 1 (PAR1)) as an integral facet of this machine with roles in regulating neurite growth through a growth factor- and cholesterol-dependent mechanism.

Sat May 01 00:00:00 UTC 2021

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

Sat May 01 00:00:00 UTC 2021

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

Sat May 01 00:00:00 UTC 2021

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

Sat May 01 00:00:00 UTC 2021

Transcriptional and Histochemical Signatures of Bone Marrow Mononuclear Cell-Mediated Resolution of Synovitis.

Frontiers in immunology

Menarim BC,El-Sheikh Ali H,Loux SC,Scoggin KE,Kalbfleisch TS,MacLeod JN,Dahlgren LA

PA5-29488 was used in Immunohistochemistry (Paraffin) to study differential transcriptional signatures of bone marrow mononuclear cells in response to normal and inflamed synovial fluid.

Thu Feb 10 00:00:00 UTC 2022

Transcriptional and Histochemical Signatures of Bone Marrow Mononuclear Cell-Mediated Resolution of Synovitis.

Frontiers in immunology

Menarim BC,El-Sheikh Ali H,Loux SC,Scoggin KE,Kalbfleisch TS,MacLeod JN,Dahlgren LA

PA5-29488 was used in Immunohistochemistry (Paraffin) to study differential transcriptional signatures of bone marrow mononuclear cells in response to normal and inflamed synovial fluid.

Thu Feb 10 00:00:00 UTC 2022

生物信息学

蛋白别名: 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase; 3-hydroxy-3-methylglutaryl coenzyme A synthase; 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble); 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble); HMG-CoA synthase; Hydroxymethylglutaryl-CoA synthase, cytoplasmic

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基因别名: B130032C06Rik; HMGCS; HMGCS1

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UniProt ID: (Human) Q01581, (Mouse) Q8JZK9

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Entrez Gene ID: (Human) 3157, (Mouse) 208715

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功能

hydroxymethylglutaryl-CoA synthase activity drug binding isomerase activity protein homodimerization activity organic acid binding catalytic activity transferase activity

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