Invitrogen

HNF4A Monoclonal Antibody (K9218)

HNF4A Antibody in Immunocytochemistry (ICC/IF)

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HNF4A Antibody (MA1-199) in ICC/IF

Immunofluorescence analysis of HNF4A was performed using 70% confluent log phase HepG2 and HeLa cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Anti-HNF4A Monoclonal Antibody (Product # MA1-199) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjug... View More

HNF4A Antibody in Immunohistochemistry (IHC)

HNF4A Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

HNF4A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines tested owing to their inherent genetic constitution. Relative expression HNF4A was observed in CaCO-2 and HepG2 cell lines as compared to HCT-116 and HeLa showing no expression of HNF4A in Western Blot using HNF4A Monoclonal Antibody (Product # MA1-199). {RE}

产品信息

MA1-199

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

10-20 µg/mL

免疫细胞化学 (ICC/IF)

1:100-1:2,000

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

1-20 µg/mL

酶联免疫吸附实验 (ELISA)

0.1 µg/mL

免疫沉淀 (IP)

Assay-dependent

染色质免疫沉淀实验 (ChIP)

查看2篇已发表文章 2篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

CUT&RUN (C&R)

- -

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

K9218

抗原

Baculovirus-expressed recombinant human HNF4 alpha (aa 3-49)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

physiological saline

内含物

0.1% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

运输条件

Wet ice

AB_2633309

产品详细信息

MA1-199 detects HNF4a in human, mouse and rat samples.

靶标信息

Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is an orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine, and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Deletion of Cd44 Inhibits Metastasis Formation of Liver Cancer in Nf2-Mutant Mice.

Cells

Gerardo-Ramírez M,Giam V,Becker D,Groth M,Hartmann N,Morrison H,May-Simera HL,Radsak MP,Marquardt JU,Galle PR,Herrlich P,Straub BK,Hartmann M

MA1-199 was used in Immunocytochemistry to suggest that CD44 may be a promising target for intervening with metastatic spreading of liver cancer.

Wed Apr 26 00:00:00 UTC 2023

Deletion of Cd44 Inhibits Metastasis Formation of Liver Cancer in Nf2-Mutant Mice.

Cells

Gerardo-Ramírez M,Giam V,Becker D,Groth M,Hartmann N,Morrison H,May-Simera HL,Radsak MP,Marquardt JU,Galle PR,Herrlich P,Straub BK,Hartmann M

MA1-199 was used in Immunocytochemistry to suggest that CD44 may be a promising target for intervening with metastatic spreading of liver cancer.

Wed Apr 26 00:00:00 UTC 2023

Deletion of Cd44 Inhibits Metastasis Formation of Liver Cancer in Nf2-Mutant Mice.

Cells

Gerardo-Ramírez M,Giam V,Becker D,Groth M,Hartmann N,Morrison H,May-Simera HL,Radsak MP,Marquardt JU,Galle PR,Herrlich P,Straub BK,Hartmann M

MA1-199 was used in Immunocytochemistry to suggest that CD44 may be a promising target for intervening with metastatic spreading of liver cancer.

Wed Apr 26 00:00:00 UTC 2023

In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed.

Angiogenesis

Bonanini F,Kurek D,Previdi S,Nicolas A,Hendriks D,de Ruiter S,Meyer M,Clapés Cabrer M,Dinkelberg R,García SB,Kramer B,Olivier T,Hu H,López-Iglesias C,Schavemaker F,Walinga E,Dutta D,Queiroz K,Domansky K,Ronden B,Joore J,Lanz HL,Peters PJ,Trietsch SJ,Clevers H,Vulto P

MA1-199 was used in Immunohistochemistry to introduce a platform for routine grafting of liver and other tissues on an in vitro grown microvascular bed.

Tue Nov 01 00:00:00 UTC 2022

DevKidCC allows for robust classification and direct comparisons of kidney organoid datasets.

Genome medicine

Wilson SB,Howden SE,Vanslambrouck JM,Dorison A,Alquicira-Hernandez J,Powell JE,Little MH

MA1-199 was used in Immunohistochemistry to develop DevKidCC, an R package that classifies cell identity of kidney organoid datasets.

Tue Feb 22 00:00:00 UTC 2022

DevKidCC allows for robust classification and direct comparisons of kidney organoid datasets.

Genome medicine

Wilson SB,Howden SE,Vanslambrouck JM,Dorison A,Alquicira-Hernandez J,Powell JE,Little MH

MA1-199 was used in Immunohistochemistry to develop DevKidCC, an R package that classifies cell identity of kidney organoid datasets.

Tue Feb 22 00:00:00 UTC 2022

Deletion of Jazf1 gene causes early growth retardation and insulin resistance in mice.

Proceedings of the National Academy of Sciences of the United States of America

Lee HY,Jang HR,Li H,Samuel VT,Dudek KD,Osipovich AB,Magnuson MA,Sklar J,Shulman GI

Published figure using HNF4A monoclonal antibody (Product # MA1-199) in Western Blot

Tue Dec 06 00:00:00 UTC 2022

ZO-2/Tjp2 suppresses Yap and Wwtr1/Taz-mediated hepatocyte to cholangiocyte transdifferentiation in the mouse liver.

NPJ Regenerative medicine

Xu J,Kausalya PJ,Ong AGM,Goh CMF,Mohamed Ali S,Hunziker W

MA1-199 was used in Immunohistochemistry-immunofluorescence to show that transdifferentiation is regulated at multiple levels and the type of injury inflicted on the Tjp2 deficient liver plays an important role in the resulting pathophysiology.

Fri Sep 23 00:00:00 UTC 2022

ZO-2/Tjp2 suppresses Yap and Wwtr1/Taz-mediated hepatocyte to cholangiocyte transdifferentiation in the mouse liver.

NPJ Regenerative medicine

Xu J,Kausalya PJ,Ong AGM,Goh CMF,Mohamed Ali S,Hunziker W

Fri Sep 23 00:00:00 UTC 2022

SARS-CoV-2 infection of human pluripotent stem cell-derived liver organoids reveals potential mechanisms of liver pathology.

iScience

Richards A,Friesen M,Khalil A,Barrasa MI,Gehrke L,Jaenisch R

MA1-199 was used in Functional Assay to recapitulate and characterize liver pathology following virus exposure.

Fri Oct 21 00:00:00 UTC 2022

Establishment of Human Leukocyte Antigen-Mismatched Immune Responses after Transplantation of Human Liver Bud in Humanized Mouse Models.

Cells

Mori A,Murata S,Tashiro N,Tadokoro T,Okamoto S,Otsuka R,Wada H,Murata T,Takahashi T,Seino KI,Taniguchi H

MA1-199 was used in Immunohistochemistry to generate immune-compromised nonobese diabetic/Shi-scid, IL-2RγKO Jic (NOG) with a homozygous expression of human leukocyte antigen (HLA) class I heavy chain (NOG-HLA-A2Tg) mice to elicit human T-cell immune responses.

Tue Feb 23 00:00:00 UTC 2021

Enhanced expression of HNF4α during intestinal epithelial differentiation is involved in the activation of ER stress.

The FEBS journal

Tunçer S,Sade-Memişoğlu A,Keşküş AG,Sheraj I,Güner G,Akyol A,Banerjee S

MA1-199 was used in ChIP assay to link differentiation to cellular stress responses, and highlights the importance of transcription factor signaling and cross-talk between the cellular events in the regulation of intestinal cell differentiation.

Mon Jun 01 00:00:00 UTC 2020

An Unexpected Role of Cholesterol Sulfotransferase and its Regulation in Sensitizing Mice to Acetaminophen-Induced Liver Injury.

Molecular pharmacology

An Y,Wang P,Xu P,Tung HC,Xie Y,Kirisci L,Xu M,Ren S,Tian X,Ma X,Xie W

MA1-199 was used in Western Blotting to study the role of SULT2B1b in acetaminophen toxicity.

Sat Jun 01 00:00:00 UTC 2019

Regulation of Cholesterol Sulfotransferase SULT2B1b by Hepatocyte Nuclear Factor 4α Constitutes a Negative Feedback Control of Hepatic Gluconeogenesis.

Molecular and cellular biology

Bi Y,Shi X,Zhu J,Guan X,Garbacz WG,Huang Y,Gao L,Yan J,Xu M,Ren S,Ren S,Liu Y,Ma X,Li S,Xie W

MA1-199 was used in Western blotting following immunoprecipitation to investigate if the SULT2B1b gene is a transcriptional target of hepatocyte nuclear factor 4α (HNF4α), and if the induction of SULT2B1b by HNF4α represents a negative feedback to limit the gluconeogenic activity of HNF4α.

Sun Apr 01 00:00:00 UTC 2018

Regulation of Cholesterol Sulfotransferase SULT2B1b by Hepatocyte Nuclear Factor 4α Constitutes a Negative Feedback Control of Hepatic Gluconeogenesis.

Molecular and cellular biology

Bi Y,Shi X,Zhu J,Guan X,Garbacz WG,Huang Y,Gao L,Yan J,Xu M,Ren S,Ren S,Liu Y,Ma X,Li S,Xie W

Sun Apr 01 00:00:00 UTC 2018

Regulation of Cholesterol Sulfotransferase SULT2B1b by Hepatocyte Nuclear Factor 4α Constitutes a Negative Feedback Control of Hepatic Gluconeogenesis.

Molecular and cellular biology

Bi Y,Shi X,Zhu J,Guan X,Garbacz WG,Huang Y,Gao L,Yan J,Xu M,Ren S,Ren S,Liu Y,Ma X,Li S,Xie W

Sun Apr 01 00:00:00 UTC 2018

生物信息学

蛋白别名: alpha transcription factor 4; FLJ39654; hepatic nuclear factor 4 alpha; hepatic nuclear factor 4, alpha; hepatocyte nuclear factor 4, alpha; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; HNF4 alpha; HNF4-alpha; HNF4alpha10; HNF4alpha10/11/12; HNF4alpha11; HNF4alpha12; Nuclear receptor 2A1; Nuclear receptor subfamily 2 group A member 1; TCF-14; Transcription factor 14; Transcription factor HNF-4

查看更多收起

基因别名: FRTS4; HNF-4; HNF4; HNF4A; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14

查看更多收起

UniProt ID: (Human) P41235, (Mouse) P49698, (Rat) P22449

查看更多收起

Entrez Gene ID: (Human) 3172, (Mouse) 15378, (Rat) 25735

查看更多收起

功能

fatty-acyl-CoA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding receptor binding fatty acid binding protein binding zinc ion binding palmitoyl-CoA hydrolase activity protein homodimerization activity transcription regulatory region DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor binding sequence-specific DNA binding metal ion binding repressing transcription factor binding C4 zinc finger nuclear receptor

参与通路

regulation of transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter ornithine metabolic process lipid metabolic process acyl-CoA metabolic process xenobiotic metabolic process establishment of tissue polarity sex differentiation blood coagulation negative regulation of cell proliferation response to glucose regulation of gastrulation regulation of lipid metabolic process signal transduction involved in regulation of gene expression cell differentiation negative regulation of cell growth intracellular receptor signaling pathway regulation of microvillus assembly negative regulation of protein import into nucleus, translocation response to drug negative regulation of tyrosine phosphorylation of Stat5 protein glucose homeostasis steroid hormone mediated signaling pathway negative regulation of sequence-specific DNA binding transcription factor activity cell-cell junction organization positive regulation of gluconeogenesis positive regulation of fatty acid biosynthetic process positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter regulation of insulin secretion response to cAMP lipid homeostasis phospholipid homeostasis SMAD protein signal transduction triglyceride homeostasis negative regulation of activation of JAK2 kinase activity positive regulation of cholesterol homeostasis transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter

HNF4A Monoclonal Antibody (K9218)

HNF4A Antibody (MA1-199) in ICC/IF

产品信息

MA1-199

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

功能检测 (Functional)

CUT&RUN (C&R)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

HNF4A Monoclonal Antibody (K9218)

HNF4A Antibody (MA1-199) in ICC/IF

产品信息

MA1-199

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

功能检测 (Functional)

CUT&RUN (C&R)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)