Invitrogen

HNF4A Recombinant Rabbit Monoclonal Antibody (SN72-03)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-32295

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:3000
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:1000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SN72-03

Immunogen

Synthetic peptide within Human HNF-4-alpha aa 21-70
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809580

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Hepatocyte nuclear factor 4 alpha (HNF4 alpha) is an orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine, and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ39654; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4, alpha; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; HNF4 alpha; HNF4-alpha; HNF4alpha10; HNF4alpha10/11/12; HNF4alpha11; HNF4alpha12; Nuclear receptor 2A1; Nuclear receptor subfamily 2 group A member 1; TCF-14; Transcription factor 14; Transcription factor HNF-4

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Gene Aliases: FRTS4; HNF-4; HNF4; HNF4A; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14

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UniProt ID: (Human) P41235, (Mouse) P49698

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Entrez Gene ID: (Human) 3172, (Mouse) 15378

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Function(s)
fatty-acyl-CoA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding receptor binding fatty acid binding protein binding zinc ion binding palmitoyl-CoA hydrolase activity protein homodimerization activity transcription regulatory region DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor binding sequence-specific DNA binding metal ion binding repressing transcription factor binding C4 zinc finger nuclear receptor
Process(es)
regulation of transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter ornithine metabolic process lipid metabolic process acyl-CoA metabolic process xenobiotic metabolic process establishment of tissue polarity sex differentiation blood coagulation negative regulation of cell proliferation response to glucose regulation of gastrulation regulation of lipid metabolic process signal transduction involved in regulation of gene expression cell differentiation negative regulation of cell growth intracellular receptor signaling pathway regulation of microvillus assembly negative regulation of protein import into nucleus, translocation response to drug negative regulation of tyrosine phosphorylation of Stat5 protein glucose homeostasis steroid hormone mediated signaling pathway negative regulation of sequence-specific DNA binding transcription factor activity cell-cell junction organization positive regulation of gluconeogenesis positive regulation of fatty acid biosynthetic process positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter regulation of insulin secretion response to cAMP lipid homeostasis phospholipid homeostasis SMAD protein signal transduction triglyceride homeostasis negative regulation of activation of JAK2 kinase activity positive regulation of cholesterol homeostasis transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter
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