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HOXB3 Antibody (PA5-103890) in ICC/IF
Immunocytochemistry analysis of HOXB3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with HOXB3 polyclonal antibody (Product # PA5-103890) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103890
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HOXB3(Accession P14651), corresponding to amino acid residues A359-D409.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853222
Product Specific Information
Antibody detects endogenous levels of total HOXB3.
Target Information
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: homeo box 2G; homeo box B3; Homeobox protein Hox-2.7; Homeobox protein Hox-2G; Homeobox protein Hox-B3; Homeobox protein MH-23
Gene Aliases: Hox-2.7; HOX2; HOX2G; Hoxb-3; HOXB3
UniProt ID: (Human) P14651, (Mouse) P09026
Entrez Gene ID: (Human) 3213, (Mouse) 15410, (Rat) 303488
negative regulation of transcription from RNA polymerase II promoter
hematopoietic progenitor cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
anterior/posterior pattern specification
rhombomere development
glossopharyngeal nerve morphogenesis
thyroid gland development
embryonic skeletal system morphogenesis
regulation of neurogenesis
cartilage development
definitive hemopoiesis
face development
angiogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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