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HP1 alpha Antibody (PA5-29879) in ICC/IF
HP1 alpha Polyclonal Antibody detects HP1 alpha protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HP1 alpha protein stained by HP1 alpha Polyclonal Antibody (Product # PA5-29879) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
产品信息
PA5-29879
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein fragment corresponding to a region within amino acids 1 and 138 of Human HP1 alpha
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2547353
产品详细信息
Recommended positive controls: HeLa, HeLa nucleus.
Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (80%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Antigen p25; Cbx5 antibody; chromobox homolog 5; chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox protein homolog 5; epididymis luminal protein 25; heterochromatin protein 1 alpha; Heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HGNC:1555; HP-1 alpha antibody; HP1 alpha; HP1 alpha homolog; HP1-ALPHA; HP1Hs alpha; HP1Hs-alpha; mHP1(alpha)
基因别名: 2610029O15Rik; C75991; CBX5; HEL25; HP1; HP1A; Hp1alpha
UniProt ID: (Human) P45973, (Mouse) Q61686
Entrez Gene ID: (Human) 23468, (Mouse) 12419
