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HPRT1 Antibody (PA5-95381) in WB
Western blot analysis of HPRT/HPRT1 in, Lane 1: human Hela whole cell lysates, Lane 2: human Colo320 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse brain tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were tr... View More
Product Details
PA5-95381
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human HPRT recombinant protein (Position: A2-S110).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807184
Product Specific Information
Human HPRT shares 98.1% and 96.3% amino acid (aa) sequence identity with mouse and rat HPRT, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human Colo320 whole cell, human HepG2 whole cell, human 293T whole cell, rat brain tissue, rat spleen tissue, mouse brain tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGPRT; HGPRTase; HPRT 1; HPRT B; hypoxanthine guanine phosphoribosyl transferase 1; hypoxanthine phosphoribosyl transferase; Hypoxanthine-guanine phosphoribosyltransferase; hypoxanthine-guanine phosphoribosyltransferase 1; testicular tissue protein Li 89
Gene Aliases: C81579; HGPRT; Hgprtase; HPGRT; HPRT; HPRT1
UniProt ID: (Human) P00492, (Mouse) P00493, (Rat) P27605
Entrez Gene ID: (Human) 3251, (Mouse) 15452, (Rat) 24465
response to amphetamine
purine nucleotide biosynthetic process
purine ribonucleoside salvage
adenine salvage
guanine salvage
grooming behavior
locomotory behavior
nucleoside metabolic process
cytolysis
striatum development
cerebral cortex neuron differentiation
central nervous system neuron development
GMP salvage
IMP salvage
dopamine metabolic process
hypoxanthine salvage
positive regulation of dopamine metabolic process
GMP catabolic process
IMP metabolic process
adenine metabolic process
hypoxanthine metabolic process
lymphocyte proliferation
dendrite morphogenesis
protein homotetramerization
spermatogenesis
brain development
cellular response to insulin stimulus
purine-containing compound salvage
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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