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HRI Antibody (MA5-55267) in WB
Western blot analysis of lysates from wild type (WT) and EIF2AK1 knockdown (KD) 293T cells, using HRI Recombinant Monoclonal Antibody (Product # MA5-55267) at 1:2,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
MA5-55267
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
1G4Z2
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 337-586 of human EIF2AK1.
偶联物
形式
Liquid
浓度
2.79 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
内含物
0.05% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
产品详细信息
Immunogen Sequence: SIVEQQLPLR RNSHLEESFT STEESSEENV NFLGQTEAQY HLMLHIQMQL CELSLWDWIV ERNKRGREYV DESACPYVMA NVATKIFQEL VEGVFYIHNM GIVHRDLKPR NIFLHGPDQQ VKIGDFGLAC TDILQKNTDW TNRNGKRTPT HTSRVGTCLY ASPEQLEGSE YDAKSDMYSL GVVLLELFQP FGTEMERAEV LTGLRTGQLP ESLRKRCPVQ AKYIQHLTRR NSSQRPSAIQ LLQSELFQNS
靶标信息
EIF2AK1 (HRI) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK1, signaling is initiated by low levels of heme. The HRI gene is localized to 7p22 where its 3' end slightly overlaps the 3' end of the gene JTV1. The two genes are transcribed from opposite strands and kinase activity is induced by low levels of heme and inhibited by the presence of heme.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Eukaryotic translation initiation factor 2-alpha kinase 1; HCR; he; heme regulated initiation factor 2 alpha kinase; heme sensitive initiation factor 2a kinase; Heme-controlled repressor; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; heme-regulated initiation factor 2-alpha kinase; heme-regulated repressor; Hemin-sensitive initiation factor 2-alpha kinase; hHRI
基因别名: EIF2AK1; HCR; HRI; KIAA1369; PRO1362
UniProt ID: (Human) Q9BQI3
Entrez Gene ID: (Human) 27102
acute inflammatory response
phagocytosis
response to stress
negative regulation of cell proliferation
response to external stimulus
regulation of translational initiation by eIF2 alpha phosphorylation
regulation of eIF2 alpha phosphorylation by heme
macrophage differentiation
negative regulation of translational initiation by iron
protoporphyrinogen IX metabolic process
protein autophosphorylation
negative regulation of hemoglobin biosynthetic process
iron ion homeostasis
