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FIGURE: 1 / 1
HS2ST1 Antibody (PA5-89472) in WB
Western blot analysis of HS2ST1 in extracts of various cell lines. Samples were incubated with HS2ST1 Polyclonal antibody (Product # PA5-89472) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89472
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-229 of human HS2ST1 (NP_0011279641)
Conjugate
Form
Liquid
Concentration
1.69 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805591
Product Specific Information
Immunogen sequence: MGLLRIMMPP KLQLLAVVAF AVAMLFLENQ IQKLEESRSK LERAIARHEV REIEQRHTMD GPRQDATLDE EEDMVIIYNR VPKTASTSFT NIAYDLCAKN KYHVLHINTT KNNPVMSLQD QVRFVKNITS WKEMKPGFYH GHVSYLDFAK FGVKKKPIYI NVIRDPIERL VSYYYFLRFG DDYRPGLRRR KQGDKKTFDE CVAEGGSDCA PEKLWLQIPF FCGHSSECW; Positive Samples: U-87MG, Mouse lung, Rat thymus; Cellular Location: Golgi apparatus membrane, Single-pass type II membrane protein
Target Information
Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-O-sulfotransferase; 2-OST; 2OST; Heparan sulfate 2-O-sulfotransferase 1
Gene Aliases: AW214369; dJ604K5.2; HS2ST; HS2ST1; KIAA0448; mKIAA0448
UniProt ID: (Human) Q7LGA3, (Mouse) Q8R3H7
Entrez Gene ID: (Human) 9653, (Rat) 292155, (Mouse) 23908
heparan sulfate 2-O-sulfotransferase activity
N-acetylglucosamine 6-O-sulfotransferase activity
N-acetylgalactosamine 4-O-sulfotransferase activity
sulfotransferase activity
HNK-1 sulfotransferase activity
transferase activity
heparan sulfate 6-O-sulfotransferase activity
trans-9R,10R-dihydrodiolphenanthrene sulfotransferase activity
1-phenanthrol sulfotransferase activity
3-phenanthrol sulfotransferase activity
4-phenanthrol sulfotransferase activity
trans-3,4-dihydrodiolphenanthrene sulfotransferase activity
9-phenanthrol sulfotransferase activity
2-phenanthrol sulfotransferase activity
phenanthrol sulfotransferase activity
1-hydroxypyrene sulfotransferase activity
galactose 3-O-sulfotransferase activity
proteoglycan sulfotransferase activity
cholesterol sulfotransferase activity
hydroxyjasmonate sulfotransferase activity
transferase
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