Invitrogen

HSC70 Monoclonal Antibody (BB70)

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HSC70 Antibody (MA1-10891) in WB

Western blot analysis of rat liver and fish ventricle lysates using Heat Shock Protein 70/HSC Protein 7 Monoclonal Antibody (Product # MA1-10891). Whole tissue extracts from rat liver (20 µg) and Chaenocephalus aceratus and Notothenia coriiceps ventricles (200 µg) were separated on 10% SDS-PAGE gels and transferred to nitrocellulose membranes. Following transfer, membranes were blocked with 5% milk in PBST and probed with Heat Shock Protein 70/HSC Protein 7 Monoclonal Antibody (Product # MA1-10891) at 1:2000 dilution followed by donkey ant... View More

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产品信息

MA1-10891

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

免疫组化 (IHC)

5 µg/mL

免疫沉淀 (IP)

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产品规格

种属反应

Chicken, Human, Many, Mouse, Rat

已发表种属

Rabbit

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

BB70

抗原

Chicken Hsp70/Hsp90 complex.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2194138

产品详细信息

MA1-10891 detects Heat Shock Protein 70/Heat Shock Cognate Protein 70 in human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken, yeast, fish and Xenopus samples.

MA1-10891 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, MA1-10891 is specific for the ~72 kDa Heat Shock Protein 70/Heat Shock Cognate Protein 70 protein.

MA1-10891 immunogen is chicken Hsp70/Hsp90 complex.

靶标信息

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly.

Molecular and cellular biology

Prapapanich V,Chen S,Smith DF

MA1-10891 was used in immunoprecipitation to investigate the effect of Hip on progesterone receptor assembly

Sun Feb 01 00:00:00 UTC 1998

生物信息学

蛋白别名: AAG2; constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kD protein 8; heat shock 70kDa protein 8; heat shock cognate 70; Heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; HIP; LAP; LAP-1; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; Protein FAM10A1; SNC6

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基因别名: 2410008N15Rik; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; Hsc73; HSP71; HSP73; HSPA10; HSPA8; LAP-1; LAP1; NIP71