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HSD3B2 Antibody (PA5-106895) in ICC/IF
Immunocytochemistry analysis of HSD3B2 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with HSD3B2 polyclonal antibody (Product # PA5-106895) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106895
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Avian,
Human,
Mouse,
Rat
Published species
Chicken
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HSD3B2(Accession P26439), corresponding to amino acid residues L108-K158.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854559
Product Specific Information
Antibody detects endogenous levels of total HSD3B2.
Target Information
3 beta-hydroxysteroid dehydrogenase (3beta-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis of all classes of hormonal steroids. Two human 3beta-HSD proteins, designated type I (3beta-HSD) and type II (3beta-HSD2), are expressed by different genes and function in different areas of the body. Localized to the membrane of the endoplasmic reticulum (ER) and expressed in skin and placenta, 3beta-HSD is the type I protein that catalyzes the oxidative conversion of delta5-ene-3-beta-hydroxy steroid, as well as the conversion of various ketosteroids. Defects in the gene encoding 3beta-HSD are associated with classic salt wasting, genital ambiguity, hypogonadism, insulin-resistant polycystic ovary syndrome (PCOS) and an increased susceptibility to prostate cancer. Additionally, congenital deficiency of 3beta-HSD activity results in a severe depletion of steroid formation which can be lethal in young children.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 3 beta HSD II; 3 beta-HSD type II; 3 beta-hydroxysteroid dehydrogenase 2; 3 beta-hydroxysteroid dehydrogenase 6; 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 4; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type IV; 3-beta-HSD adrenal and gonadal type; 3-beta-HSD II; 3-beta-HSD IV; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3beta-HSD; delta 5-delta 4-isomerase type II; HSD-3beta; Hydroxy-delta-5-steroid dehydrogenase 3 beta- and steroid delta-isomerase; hydroxysteroid dehydrogenase-2, delta<5>-3-beta; hydroxysteroid dehydrogenase-6 delta<5>-3-beta; progesterone reductase; short chain dehydrogenase/reductase family 11E, member 2
Gene Aliases: HSD3B; Hsd3b1; HSD3B2; Hsd3b6; HSDB; HSDB3B; SDR11E2
UniProt ID: (Human) P26439, (Mouse) P26149, (Rat) Q62878
Entrez Gene ID: (Human) 3284, (Mouse) 15493, (Rat) 29632
steroid biosynthetic process
androgen biosynthetic process
glucocorticoid biosynthetic process
mineralocorticoid biosynthetic process
oxidation-reduction process
metabolic process
C21-steroid hormone biosynthetic process
response to lead ion
hippocampus development
Leydig cell differentiation
response to gonadotropin
negative regulation of iron ion transport
response to cadmium ion
response to corticosterone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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