Bethyl Laboratories

HSF1 Polyclonal Antibody

HSF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HSF1 Antibody (A303-175A) in IHC (P)

Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSF1 (Product # A303-174A) (Lot 1) used at a dilution of 1:5,000 (0.2 µg/mL) (left), (Product # A303-175A) (Lot 1) used at a dilution of 1:5,000 (0.2 µg/mL) (center) and (Product # A303-176A) (Lot 1) used at a dilution of 1:1,000 (0.2 µg/mL) (right). Detection: DAB.

HSF1 Antibody in Immunoprecipitation (IP)

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产品信息

A303-175A

应用

建议稀释比

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免疫印迹 (WB)

Assay-dependent

免疫组化 (IHC)

1:500-1:5,000

免疫组化（石蜡） (IHC (P))

1:500-1:5,000

免疫沉淀 (IP)

2-10 µg/mg lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residue 425 and 475 of human Heat Shock Transcription Factor 1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

tris citrate/phosphate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

靶标信息

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。