Invitrogen

HSP27 Polyclonal Antibody

HSP27 Antibody in Immunocytochemistry (ICC/IF)

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HSP27 Antibody (PA1-017) in ICC/IF

Immunofluorescent analysis of Heat Shock Protein 27 (HSP27) (green) in HeLa cells and negative control NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with HSP27 polyclonal antibody (Product # PA1-017), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product # 35552... View More

HSP27 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP27 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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HSP27 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of HSP27 was observed in MCF-7, MDA-MB-231, PC-3, DU 145, HeLa and LNCaP in comparison to HT-1080 using Anti-HSP27 Polyclonal Antibody (Product # PA1-017) in Western Blot. {RE}

产品信息

PA1-017

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

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免疫组化（石蜡） (IHC (P))

4 µg/mL

免疫细胞化学 (ICC/IF)

Assay-dependent

免疫沉淀 (IP)

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其他PubMed (MISC)

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产品规格

种属反应

Human, Non-human primate, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues L(10) L R G P S W D P F R C(21) of human Heat Shock Protein 27.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2120942

产品详细信息

PA1-017 detects heat shock protein 27(hsp27) from rat, mouse, monkey, and human tissue tissues.

PA1-017 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunohistochemistry procedures. By Western blot, this antibody detects a ~27 kDa protein which corresponds to hsp27 from HeLa cells. Immunocytochemical staining of hsp27 in HeLa cells with this antibody results in diffuse cytoplasmic staining before heat shock and diffuse cytoplasmic along with punctate staining patterns after.

The PA1-017 immunogen is a synthetic peptide corresponding to residues L(10) L R G P S W D P F R C(21) of human Heat Shock Protein 27. This peptide is 81% conserved in mice, rats, and hamsters. The phosphorylated peptide (Cat. # PEP-187) and non-phosphorylated peptide (Cat. # PEP-240) are available for use in neutralization and control experiments.

靶标信息

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The Glyoxalase System Is a Novel Cargo of Amniotic Fluid Stem-Cell-Derived Extracellular Vesicles.

Antioxidants (Basel, Switzerland)

Romani R,Talesa VN,Antognelli C

PA1-017 was used in Western Blotting and Immunoprecipitaiton to investigate whether MG-derived MG-H1, as well as glyoxalases, could be novel molecule cargo in extracellular vesicles.

Fri Aug 05 00:00:00 UTC 2022

The Glyoxalase System Is a Novel Cargo of Amniotic Fluid Stem-Cell-Derived Extracellular Vesicles.

Antioxidants (Basel, Switzerland)

Romani R,Talesa VN,Antognelli C

PA1-017 was used in Western Blotting and Immunoprecipitaiton to investigate whether MG-derived MG-H1, as well as glyoxalases, could be novel molecule cargo in extracellular vesicles.

Fri Aug 05 00:00:00 UTC 2022

The Glyoxalase System Is a Novel Cargo of Amniotic Fluid Stem-Cell-Derived Extracellular Vesicles.

Antioxidants (Basel, Switzerland)

Romani R,Talesa VN,Antognelli C

PA1-017 was used in Western Blotting and Immunoprecipitaiton to investigate whether MG-derived MG-H1, as well as glyoxalases, could be novel molecule cargo in extracellular vesicles.

Fri Aug 05 00:00:00 UTC 2022

Heat Shock Protein A6 Is Especially Involved in Enterovirus 71 Infection.

Frontiers in microbiology

Jia J,Liu G,Zhong J,Yan R,Song X,Zheng K,Ren Z,He Z,Zhu Q

Published figure using HSP27 polyclonal antibody (Product # PA1-017) in Western Blot

Tue Mar 22 00:00:00 UTC 2022

Small heat shock protein 27 (Hsp27) expression is highly induced in rat myometrium during late pregnancy and labour.

Reproduction (Cambridge, England)

White BG,Williams SJ,Highmore K,Macphee DJ

PA1-017 was used in immunohistochemistry and western blot to examine the expression of Hsp27 in rat myometrium during late pregnancy and labour

Sat Jan 01 00:00:00 UTC 2005

生物信息学

蛋白别名: 28 kDa heat shock protein; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; HS; HSP; HSP 27; HspB1; Phospho-HSP 27; SRP27; Stress-responsive protein 27

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基因别名: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27

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UniProt ID: (Human) P04792

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Entrez Gene ID: (Human) 3315, (Rat) 24471

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功能

protein kinase C binding protein binding protein kinase C inhibitor activity protein kinase binding identical protein binding ubiquitin binding poly(A) RNA binding

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