NeoBiotechnologies

HSP27 (Heat Shock Protein 27) Monoclonal Antibody (HSPB1, 774)

HSP27 (Heat Shock Protein 27) Antibody in Immunocytochemistry (ICC/IF)

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HSP27 (Heat Shock Protein 27) Antibody (3315-MSM2-P1) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of PFA-fixed MCF-7 cells using HSP27 (Heat Shock Protein 27) Monoclonal Antibody (Product # 3315-MSM2-P1) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

HSP27 (Heat Shock Protein 27) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HSP27 (Heat Shock Protein 27) Antibody in Western Blot (WB)

HSP27 (Heat Shock Protein 27) Antibody in Flow Cytometry (Flow)

HSP27 (Heat Shock Protein 27) Antibody in SDS-PAGE (SDS-PAGE)

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产品信息

3315-MSM2-P1

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

免疫细胞化学 (ICC/IF)

1-2 µg/mL

流式细胞分析 (Flow)

1-2 µg/10^6 cells

产品规格

种属反应

Chicken, Chimpanzee, Human, Mouse, Non-human primate, Sheep, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

HSPB1, 774

抗原

Recombinant human HSPB1 protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

200 µg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4, with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

靶标信息

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: 25 kDa IAP; 27 kDa heat shock protein 1; 28 kDa heat shock protein; Actin polymerization inhibitor; DKFZp586P1322; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; Heat shock protein beta-1; Heat shock protein family B member 1; heat shock protein, 25 kDa; HS; HSP; HSP 25; HSP 27; HspB1; inhibitor of actin polymerization; p25; Phospho-HSP 27; SRP27; Stress-responsive protein 27; truncated hsp25

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基因别名: 27kDa; CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; HSPB1; SRP27

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UniProt ID: (Human) P04792, (Chicken) Q00649, (Mouse) P14602