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HSP60 Antibody (MA5-45109) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using HaCaT cells. Fixation involved Cold 100% methanol at -20°C for 10 minutes. Samples were incubated with Hsp60 monoclonal antibody (Product # MA5-45109) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.
产品信息
MA5-45109
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Dog,
Chicken,
Fruit fly,
Guinea pig,
Hamster,
Human,
Insect,
Mouse,
Non-human primate,
Sheep,
Plant,
Pig,
Rabbit,
Rat,
Shrew,
Xenopus
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
LK1
抗原
Recombinant human HSP60
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.46mM phosphate/2.48mM MES with 2mM EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931563
产品详细信息
0.05 µg/mL of MA5-45109 was sufficient for detection of HSP60 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody. |Detects approximately 60kDa.
靶标信息
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; 60-kDa heat shock protein, mitochondrial; CG12101-PA; CG12101-PB; chaperonin; Chaperonin 60; CPN60; heat shock 60 kDa protein 1; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein; heat shock protein 1 (chaperonin); Heat shock protein 60; heat shock protein 60 (liver); heat shock protein 60 kDa; Heat shock protein 60A; heat shock protein 65; Heat shock protein family D member 1; heat shock protein, 60 kDa; HSP; Hsp 60; HSP-60; HSP-65; hsp60; Hsp60A-PA; Hsp60A-PB; Hsp60s1; HuCHA60; lethal(1)10Ac; mitochondrial heat shock 60 kDa protein 1; Mitochondrial matrix protein P1; P1 protein; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1
基因别名: 12; 60kDa; BP5; CG12101; chaperonin; CPN60; d-hsp60; Dm10A; Dmel\CG12101; Dmel_CG12101; DmHsp60; G62; GROEL; HLD4; Hsp 60; HSP-60; HSP60; HSP60A; Hsp64; HSP65; HSPD1; Hspd1-30p; HuCHA60; I79_011398; IEF16; l(1)10Ac; l(1)BP5; l(1)dp025; l(1)G8; l(1)HM21; l(1)L12; Mmp-P1; RCJMB04_7g5; SPG13; SSP 7506; XELAEV_18045048mg
ATP binding
isomerase activity
lipopolysaccharide binding
p53 binding
double-stranded RNA binding
protein binding
high-density lipoprotein particle binding
ubiquitin protein ligase binding
apolipoprotein A-I binding
chaperone binding
DNA replication origin binding
single-stranded DNA binding
ATPase activity
poly(A) RNA binding
unfolded protein binding
nucleotide binding
protease binding
protein complex binding
insulin binding
protein heterodimerization activity
misfolded protein binding
B cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
positive regulation of T cell mediated immune response to tumor cell
protein folding
activation of cysteine-type endopeptidase activity involved in apoptotic process
apoptotic mitochondrial changes
response to cold
positive regulation of interferon-alpha production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
mitochondrial unfolded protein response
protein refolding
B cell proliferation
T cell activation
positive regulation of macrophage activation
negative regulation of apoptotic process
protein import into mitochondrial intermembrane space
isotype switching to IgG isotypes
protein stabilization
positive regulation of T cell activation
interaction with symbiont
cellular response to interleukin-7
detection of misfolded protein
response to unfolded protein
B cell activation
positive regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of inflammatory response
chaperone mediated protein folding requiring cofactor
'de novo' protein folding
viral process
chaperone-mediated protein complex assembly
protein maturation
protein targeting to mitochondrion
mitochondrion organization
response to heat
cellular response to heat
response to hypoxia
response to organic substance
response to organic cyclic compound
response to lipopolysaccharide
response to ATP
response to cocaine
response to drug
response to hydrogen peroxide
response to estrogen
