Invitrogen

HSP90 Monoclonal Antibody (4F3.E8), PerCP

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产品信息

MA5-45185

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:2,000
-

免疫组化（石蜡） (IHC (P))

1:1,000
-

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:100,000
-

免疫细胞化学 (ICC/IF)

1:100
-

酶联免疫吸附实验 (ELISA)

Assay-dependent
-

免疫沉淀 (IP)

Assay-dependent
-
产品规格

种属反应

Human, Mouse, Plant, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

4F3.E8

抗原

Recombinant Human HSP90 purified from E.coli
查看免疫原

偶联物

PerCP PerCP PerCP

激发/发射光谱

482/675 nm 查看光谱 spectra

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2931639

产品详细信息

0.5 µg/mL of MA5-45185 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 90kDa. This antibody detects both alpha and beta forms of HSP90 equally well.

靶标信息

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 and mitochondria (Chen et al., 2005 ).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 84 kDa; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein 1, beta; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; heat shock 90kDa protein 1, beta; Heat shock protein; heat shock protein 1, alpha; heat shock protein 1, beta; heat shock protein 86; heat shock protein 90 kDa; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha family class A member 1; heat shock protein 90kDa alpha family class B member 1; Heat shock protein family C member 1; Heat shock protein family C member 3; Heat shock protein HSP 90-alpha; Heat shock protein HSP 90-beta; heat shock protein, 1; heat shock protein, 84 kDa 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; HSP; HSP 84; HSP 86; HSP 90; HSP84; HSP86; HSP90-beta; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; retinal degeneration slow protein; TSTA; Tumor-specific transplantation 84 kDa antigen; Tumor-specific transplantation 86 kDa antigen

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基因别名: 86kDa; 89kDa; 90kDa; AL022974; AL024080; AL024147; C81438; D6S182; EL52; HEL-S-65p; hsp4; HSP84; Hsp84-1; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90-BETA; HSP90A; HSP90AA1; HSP90AB1; HSP90B; HSP90N; HSPC1; HSPC2; HSPC3; HSPCA; HSPCAL1; HSPCAL4; HSPCB; HSPN; LAP-2; LAP2

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UniProt ID: (Human) P07900, (Human) P08238, (Mouse) P07901, (Rat) P82995, (Mouse) P11499, (Rat) P34058

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Entrez Gene ID: (Human) 3320, (Human) 3326, (Mouse) 15519, (Rat) 299331, (Mouse) 15516, (Rat) 301252

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功能
nucleotide binding UTP binding CTP binding mRNA binding protein tyrosine kinase activity protein binding ATP binding GTP binding ATPase activity sulfonylurea receptor binding protein kinase binding protein phosphatase binding MHC class II protein complex binding nitric-oxide synthase regulator activity TPR domain binding dATP binding identical protein binding protein homodimerization activity histone deacetylase binding ion channel binding poly(A) RNA binding GTPase binding Rho GDP-dissociation inhibitor binding unfolded protein binding double-stranded RNA binding protein kinase regulator activity kinase binding cadherin binding involved in cell-cell adhesion Hsp90 family chaperone
参与通路
G2/M transition of mitotic cell cycle neuron migration skeletal muscle contraction mitochondrial transport receptor-mediated endocytosis response to unfolded protein signal transduction response to heat response to cold response to salt stress positive regulation of lamellipodium assembly cardiac muscle cell apoptotic process peptidyl-tyrosine phosphorylation regulation of protein ubiquitination positive regulation of protein import into nucleus, translocation Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway protein refolding regulation of protein complex assembly protein unfolding response to estrogen protein import into mitochondrial outer membrane positive regulation of nitric oxide biosynthetic process positive regulation of cell size response to antibiotic vascular endothelial growth factor receptor signaling pathway protein stabilization regulation of nitric-oxide synthase activity chaperone-mediated protein complex assembly positive regulation of cardiac muscle contraction chaperone-mediated autophagy regulation of cellular response to heat placenta development protein folding virion attachment to host cell positive regulation of protein binding negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of phosphoprotein phosphatase activity negative regulation of neuron apoptotic process regulation of interferon-gamma-mediated signaling pathway regulation of type I interferon-mediated signaling pathway cellular response to interleukin-4 cellular response to organic cyclic compound positive regulation of protein serine/threonine kinase activity fibril organization cell-cell adhesion nitric oxide biosynthetic process response to stress positive regulation of cytotoxic T cell differentiation regulation of catalytic activity
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