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HSPA4 Antibody (PA5-29484) in ICC/IF
Immunofluorescence analysis of HSPA4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HSPA4 Polyclonal Antibody (Product # PA5-29484) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
产品信息
PA5-29484
产品规格
种属反应
Human,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 75 and 374 of Human HSP70 4
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2546960
产品详细信息
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, U87-MG, SK-N-SH, IMR32, SK-N-AS.
Predicted reactivity: Mouse (97%), Rat (96%), Xenopus laevis (87%), Dog (98%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: epididymis secretory sperm binding protein Li 5a; Heat shock 70 kDa protein 4; Heat shock 70-related protein APG-2; heat shock 70kD protein 4; heat shock 70kDa protein 4; Heat shock protein; Heat shock protein family H member 2; heat shock protein, 110 kDa; hsp70 RY; HSP70RY; Ischemia responsive 94 kDa protein
基因别名: APG-2; APG2; HEL-S-5a; HS24/P52; Hsp110; Hsp70; hsp70RY; HSPA4; HSPH2; Irp94; RY
UniProt ID: (Human) P34932, (Rat) O88600
Entrez Gene ID: (Human) 3308, (Rat) 266759
response to unfolded protein
protein import into mitochondrial outer membrane
chaperone-mediated protein complex assembly
kidney development
negative regulation of protein phosphorylation
response to heat
positive regulation of gene expression
negative regulation of gene expression
positive regulation of protein binding
negative regulation of apoptotic process
negative regulation of DNA binding
positive regulation of angiogenesis
negative regulation of cell death
