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HYOU1 Antibody (MA5-45341) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with GRP170 monoclonal antibody (Product # MA5-45341) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,000 for 60 min RT, 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X. (A) DAPI (blue) nuclear stain... View More
产品信息
MA5-45341
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
6E3-2C3
抗原
Recombinant Full length GRP170 Protein
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931795
产品详细信息
1 µg/mL of MA5-45341 was sufficient for detection of GRP170 in 20 µg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 170kDa.
靶标信息
HYOU1 belongs to the heat shock protein 70 family. The protein is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 140 kDa Ca(2+)-binding protein; 150 kDa oxygen-regulated protein; 150-kDa oxygen regulated protein; 170 kDa glucose regulated protein GRP170; 170 kDa glucose-regulated protein; calcium binding protein 140; calcium binding protein, 140 kDa; CBP-140; DKFZp686N08236; FLJ94899; FLJ97572; Grp 170; GRP-170; Heat shock protein family H member 4; HSP 12A; Hypoxia up-regulated protein 1; Orp 150; ORP-150; oxygen regulated protein (150kD)
基因别名: AI415631; Cab140; CBP-140; GRP-170; GRP170; HSP12A; HSPH4; HYOU1; ORP-150; ORP150
UniProt ID: (Human) Q9Y4L1, (Rat) Q63617, (Mouse) Q9JKR6
Entrez Gene ID: (Human) 10525, (Rat) 192235, (Mouse) 12282
response to hypoxia
response to ischemia
response to endoplasmic reticulum stress
cellular response to hypoxia
negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
negative regulation of apoptotic process
negative regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway
ER to Golgi vesicle-mediated transport
receptor-mediated endocytosis
IRE1-mediated unfolded protein response
