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Hemoglobin A (beta chain) Monoclonal Antibody (14G2.G11.F11)

Hemoglobin A (beta chain) Antibody in Western Blot (WB)

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Hemoglobin A (beta chain) Antibody (200-301-GS4) in WB

Western Blot of Mouse Anti-Hemoglobin A (beta chain) Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Load: 50ng per lane. Primary antibody: Anti-HbA antibody at 1µg/mL overnight at 4°C. Secondary antibody: Rabbit ... View More

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产品信息

200-301-GS4

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

酶联免疫吸附实验 (ELISA)

1:20,000

免疫沉淀 (IP)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a, lambda

分类

Monoclonal

类型

Antibody

克隆号

14G2.G11.F11

抗原

Anti-Hemoglobin A (beta chain) Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.04 mg/mL

纯化类型

Protein A

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Reacts specifically with human HbA beta chain isoform. This antibody does not react with the HbS form, HbF (gamma) form, HbC forms. HbA antibody cross reacts with HbA-2.

靶标信息

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon-gamma-G-gamma-A-delta-beta-3'.

仅用于科研。不用于诊断过程。未经明确授权不得转售。