Invitrogen

HuD Polyclonal Antibody

HuD Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 8

HuD Antibody (PA5-79199) in IHC (P)

Immunohistochemical analysis of ELAVL4 in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-ELAVL4 antibody (Product # PA5-79199) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Ra... View More

HuD Antibody in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) (IHC (F), IHC (P))

HuD Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

PA5-79199

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the N-terminus of human ELAVL4 (8-45aa MEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGATTDDSK).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746315

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

HuD, otherwise known as ELAV-like protein 4 or PNEM, is a protein that in humans is encoded by the ELAVL4 gene. This human gene is located at 1p34 by in situ hybridization. The HuD/ELAVL4 protein is an RNA-binding protein. ELAVL4 has specificity for 3-prime uridylate-rich untranslated regions of growth factor mRNAs. HuD is expressed only in neurons and it binds to AU-rich element-containing mRNAs. As a result of this interaction the, half-life of the transcript is increased. Additionally, HuD is important in neurons during brain development and plasticity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Myenteric Neurons Do Not Replicate in Small Intestine Under Normal Physiological Conditions in Adult Mouse.

Cellular and molecular gastroenterology and hepatology

Virtanen H,Garton DR,Andressoo JO

PA5-79199 was used in Immunohistochemistry (Paraffin), Western Blot to conclude that within 1 week under normal physiological conditions, myenteric neurons in the small intestine do not replicate.

Mon Jun 20 00:00:00 UTC 2022

Myenteric Neurons Do Not Replicate in Small Intestine Under Normal Physiological Conditions in Adult Mouse.

Cellular and molecular gastroenterology and hepatology

Virtanen H,Garton DR,Andressoo JO

PA5-79199 was used in Immunohistochemistry (Paraffin), Western Blot to conclude that within 1 week under normal physiological conditions, myenteric neurons in the small intestine do not replicate.

Mon Jun 20 00:00:00 UTC 2022

Myenteric Neurons Do Not Replicate in Small Intestine Under Normal Physiological Conditions in Adult Mouse.

Cellular and molecular gastroenterology and hepatology

Virtanen H,Garton DR,Andressoo JO

PA5-79199 was used in Immunohistochemistry (Paraffin), Western Blot to conclude that within 1 week under normal physiological conditions, myenteric neurons in the small intestine do not replicate.

Mon Jun 20 00:00:00 UTC 2022

The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons.

The Journal of biological chemistry

Bodin R,Paillé V,Oullier T,Durand T,Aubert P,Le Berre-Scoul C,Hulin P,Neunlist M,Cissé M

PA5-79199 was used in Immunohistochemistry (Paraffin), Western Blot to identify a critical role for EphB2 in the ENS and reveal a unique EphB2-mediated molecular program of synapse regulation in enteric neurons.

Mon Nov 01 00:00:00 UTC 2021

The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons.

The Journal of biological chemistry

Bodin R,Paillé V,Oullier T,Durand T,Aubert P,Le Berre-Scoul C,Hulin P,Neunlist M,Cissé M

Mon Nov 01 00:00:00 UTC 2021

生物信息学

蛋白别名: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV-like protein 4; Hu antigen D; HU-antigen D; HuD; Paraneoplastic encephalomyelitis antigen HuD; RNA-binding protein HUD3

查看更多收起

基因别名: Elav; ELAVL4; HUD; PNEM; r-HuD; RGD1561943

查看更多收起

UniProt ID: (Human) P26378, (Mouse) Q61701, (Rat) O09032

查看更多收起

Entrez Gene ID: (Human) 1996, (Mouse) 15572, (Rat) 432358

查看更多收起

功能

nucleotide binding RNA binding mRNA 3'-UTR binding AU-rich element binding nucleic acid binding mRNA binding protein binding translation initiation factor

参与通路