Invitrogen

HuR Monoclonal Antibody (3A2)

HuR Antibody in Immunocytochemistry (ICC/IF)

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HuR Antibody (MA1-167) in ICC/IF

Immunofluorescence analysis of HuR was performed using 70% confluent log phase HeLa cells treated with Actinomycin D (1uM for 19hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HuR Monoclonal Antibody (3A2) (Product # MA1-167) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Flu... View More

HuR Antibody in Immunoprecipitation (IP)

HuR Antibody in RNA Immunoprecipitation (RIP)

Product Image Gallery

HuR Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis using HuR Monoclonal Antibody (3A2) (Product # MA1-167), shows translocation from nucleus to cytoplasm in HeLa cell line when treated with Actinomycin D. {TM}

产品信息

MA1-167

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:125-1:500

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免疫细胞化学 (ICC/IF)

1:25-1:100

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免疫沉淀 (IP)

2 µg/10^6 cells

RNA免疫共沉淀 (RIP)

2 µg/10^6 cells

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3A2

抗原

Recombinant human HuR protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2-7.4, with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2536870

靶标信息

The protein encoded by this gene is a member of the ELAVL protein family. This encoded protein contains 3 RNA-binding domains and binds cis-acting AU-rich elements. It destabilizes mRNAs and thereby regulates gene expression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Long Noncoding RNA SCIRT Promotes HUVEC Angiogenesis via Stabilizing VEGFA mRNA Induced by Hypoxia.

Oxidative medicine and cellular longevity

Gao L,Yang J,Li Y,Liu K,Sun H,Tang J,Xia Z,Zhang L,Hu Z

MA1-167 was used in Western Blotting to reveal that lncRNA SCIRT can mediate endothelial angiogenesis by stabilizing the VEGFA mRNA via modulating RBP HuR stability under the HR condition.

Wed Jun 15 00:00:00 UTC 2022

The role of human antigen R (HuR) in modulating proliferation, senescence and radiosensitivity of skin cells.

Experimental and therapeutic medicine

Yu D,Feng Y,Jiang Z,Yan T,Fang K,Shi Y,Zhang J,Zhang S

MA1-167 was used in RNA Immunoprecipitation to study the key role of HuR in modulating proliferation, senescence and radiosensitivity of skin cells providing a new therapeutic strategy for cosmetic treatments and to combat skin injury.

Thu Sep 01 00:00:00 UTC 2022

Heat stress induced arginylation of HuR promotes alternative polyadenylation of Hsp70.3 by regulating HuR stability and RNA binding.

Cell death and differentiation

Deka K,Saha S

MA1-167 was used in Western Blotting to identify human antigen R (HuR) as a direct target of arginylation.

Mon Feb 01 00:00:00 UTC 2021

RNA-binding protein HuR regulates translation of vitamin D receptor modulating rapid epithelial restitution after wounding.

American journal of physiology. Cell physiology

Zhang Y,Cai JZ,Xiao L,Chung HK,Ma XX,Chen LL,Rao JN,Wang JY

MA1-167 was used in Immunocytochemistry to indicate that HuR enhances VDR translation by directly interacting with its mRNA via 3'-UTR and that induced VDR by HuR is crucial for rapid intestinal epithelial restitution after wounding.

Wed Jul 01 00:00:00 UTC 2020

生物信息学

蛋白别名: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R); ELAV like 1; Elav-like generic protein; ELAV-like protein 1; embryonic lethal, abnormal vision, drosophila, homolog-like 1; Hu antigen R; HU-antigen A; Hu-antigen R; Human antigen R; HuR; MelG

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基因别名: 2410055N02Rik; ELAV1; ELAVL1; Elra; Hua; HUR; MelG; W91709

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UniProt ID: (Human) Q15717, (Mouse) P70372, (Rat) B5DF91

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Entrez Gene ID: (Human) 1994, (Mouse) 15568, (Rat) 363854

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功能