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Invitrogen
Human Hematopoietic Lineage Antibody Cocktail, eFluor™ 450, eBioscience™
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Human Hematopoietic Lineage Antibody (22-7775-72) in Flow
Normal human peripheral blood cells were stained with Anti-Human CD34 FITC (Product # 11-0349-42), Anti-Human CD38 PE (Product # 12-0388-42), and the Human Hematopoietic Lineage eFluor® 450 Cocktail. Total viable cells were used for analysis. Cells in the right dot plot are gated on lineage negative events, depicted in the left histogram.
产品信息
22-7775-72
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
分类
Cocktail
类型
Antibody
克隆号
RPA-2.10, OKT3, 61D3, CB16, HIB19, TULY56, HIR2
偶联物
激发/发射光谱
405/445 nm
查看光谱
形式
Liquid
浓度
20 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2642588
产品详细信息
Description: The Human Hematopoietic Lineage eFluor™ 450 Cocktail contains eFluor™ 450-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.
This cocktail contains the following antibodies:
Anti-Human CD2 (RPA-2.10) eFluor™ 450
Anti-Human CD3 (OKT3) eFluor™ 450
Anti-Human CD14 (61D3) eFluor™ 450
Anti-Human CD16 (CB16) eFluor™ 450
Anti-Human CD19 (HIB19) eFluor™ 450
Anti-Human CD56 (TULY56) eFluor™ 450
Anti-Human CD235a (HIR2) eFluor™ 450
Applications Reported: Human Hematopoietic Lineage eFluor™ 450 Cocktail has been reported for use in flow cytometric analysis.
Applications Tested: Human Hematopoietic Lineage eFluor™ 450 Cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
靶标信息
Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: 17A2; Hematopoietic Lineage Cocktail; M1/70; RA3-6B2; RB6-8C5; TER-119
