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ICAD (cleaved Asp224) Antibody (PA5-77941) in WB
Western Blot using ICAD (cleaved Asp224) Polyclonal Antibody (Product # PA5-77941). Untreated (–) and treated (+) Jurkat whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ICAD (cleaved Asp224) Polyclonal Antibody (Product # PA5-77941) diluted at 1:1,000.
产品信息
PA5-77941
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ICAD (cleaved Asp224). The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.73 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2735945
产品详细信息
Positive Control: Jurkat, Jurkat (10uM etoposide treatment for 24h)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DFF-45; DFF45; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD
基因别名: DFF-45; DFF1; DFF45; DFFA; H13; ICAD
UniProt ID: (Human) O00273
Entrez Gene ID: (Human) 1676
