Invitrogen

ICAM-1 Monoclonal Antibody (15.2)

ICAM-1 Antibody in Flow Cytometry (Flow)

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ICAM-1 Antibody (MA1-80910) in Flow

Knockout of ICAM-1 (CD54) was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR845355_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD54 was performed by staining Raji CD54 Knock out cells with1 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eBioscience™ (Product # 14-4714-82, yellow histogram) or1 µgICAM-1 Monoclonal Antibody (15.2) (Product # MA1-80910, blue histogram) followed by Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary... View More

ICAM-1 Antibody in Inhibition Assays (IA)

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产品信息

MA1-80910

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组化（冰冻） (IHC (F))

1:50-1:100

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

1:50-1:100

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酶联免疫吸附实验 (ELISA)

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免疫沉淀 (IP)

Assay-dependent

中和实验 (Neu)

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抑制试剂 (IA)

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产品规格

种属反应

Human, Pig

已发表种属

Human, Pig

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

15.2

抗原

Human monocytes.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_929068

产品详细信息

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. A suggested positive control for immunohistochemical applications is human tonsil.

Mouse anti Human CD54 antibody, clone 15.2 recognizes the human CD54 cell surface antigen also known as intracellular Adhesion Molecule -1 (ICAM-1) or Major group rhinovirus receptor.CD54 is expressed by many cells following activation by inflammatory mediators.

靶标信息

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Neutrophils impose strong immune pressure against PfEMP1 variants implicated in cerebral malaria.

EMBO reports

Zelter T,Strahilevitz J,Simantov K,Yajuk O,Adams Y,Ramstedt Jensen A,Dzikowski R,Granot Z

MA1-80910 was used in Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation to provide mechanistic insight into the interactions between neutrophils and iRBCs and demonstrate the important influence of PfEMP1 on the selective innate response to cerebral malaria.

Tue Jun 07 00:00:00 UTC 2022

Neutrophils impose strong immune pressure against PfEMP1 variants implicated in cerebral malaria.

EMBO reports

Zelter T,Strahilevitz J,Simantov K,Yajuk O,Adams Y,Ramstedt Jensen A,Dzikowski R,Granot Z

Tue Jun 07 00:00:00 UTC 2022

ICAM-1 and ICAM-2 Are Differentially Expressed and Up-Regulated on Inflamed Pulmonary Epithelium, but Neither ICAM-2 nor LFA-1: ICAM-1 Are Required for Neutrophil Migration Into the Airways In Vivo.

Frontiers in immunology

Chong DLW,Rebeyrol C,José RJ,Williams AE,Brown JS,Scotton CJ,Porter JC

MA1-80910 was used in Immunohistochemistry to suggest that although β2 integrins interacting with ICAMs may regulate low levels of neutrophil traffic in healthy lung or early in inflammation when the epithelial barrier is intact; these interactions may be redundant later in inflammation when epithelial junctions are disrupted and no longer limit TEpM.

Mon Oct 25 00:00:00 UTC 2021

Use of thermodynamic coupling between antibody-antigen binding and phospholipid acyl chain phase transition energetics to predict immunoliposome targeting affinity.

Journal of liposome research

Klegerman ME,Zou Y,Golunski E,Peng T,Huang SL,McPherson DD

MA1-80910 was used in ELISA to study the use of thermodynamic analysis of antibody-antigen interactions and phospholipid phase transitions to better predict the targeting affinity of immunoliposomes

Mon Sep 01 00:00:00 UTC 2014

Nitric oxide improves molecular imaging of inflammatory atheroma using targeted echogenic immunoliposomes.

Atherosclerosis

Kim H,Kee PH,Rim Y,Moody MR,Klegerman ME,Vela D,Huang SL,McPherson DD,Laing ST

MA1-80910 was used in immunohistochemistry to study the use of NO pretreatment of targeted echogenic immunoliposomes to improve atheroma penetration and visualization in imaging studies

Sun Dec 01 00:00:00 UTC 2013

Histopathologies, immunolocalization, and a glycan binding screen provide insights into Plasmodium falciparum interactions with the human placenta.

Biology of reproduction

Hromatka BS,Ngeleza S,Adibi JJ,Niles RK,Tshefu AK,Fisher SJ

MA1-80910 was used in immunohistochemistry to study the mechanisms underlying the interaction of Plasmodium falciparum with the human placenta

Sat Jun 01 00:00:00 UTC 2013

Palm tissue displaying a polyclonal autoimmune response in patients affected by a new variant of endemic pemphigus foliaceus in Colombia, South America.

European journal of dermatology : EJD

Abreu Velez AM,Howard MS,Hashimoto T

MA1-80910 was used in immunohistochemistry to investigate pathological alterations in endemic pemphigus foliaceus in South America

Thu Apr 01 00:00:00 UTC 2010

Expression of intercellular adhesion molecule-1 in umbilical and placental vascular tissue of gestational diabetic and normal pregnancies.

Archives of gynecology and obstetrics

Kurt M,Zulfikaroglu E,Ucankus NL,Omeroglu S,Ozcan U

MA1-80910 was used in immunohistochemistry to examine the expression of intercellular adhesion molecule-1 in vascular tissue during pregnancy

Fri Jan 01 00:00:00 UTC 2010

Novel anti-inflammatory properties of the angiogenesis inhibitor vasostatin.

The Journal of investigative dermatology

Huegel R,Velasco P,De la Luz Sierra M,Christophers E,Schröder JM,Schwarz T,Tosato G,Lange-Asschenfeldt B

MA1-80910 was used in immunocytochemistry to investigate the anti-inflammatory effect of angiogenesis inhibitor vasostatin

Mon Jan 01 00:00:00 UTC 2007

Intravascular ultrasound molecular imaging of atheroma components in vivo.

Journal of the American College of Cardiology

Hamilton AJ,Huang SL,Warnick D,Rabbat M,Kane B,Nagaraj A,Klegerman M,McPherson DD

MA1-80910 was used in blocking or activating experiment to use intravascular ultrasound molecular imaging to study atheroma components in vivo

Wed Feb 04 00:00:00 UTC 2004

生物信息学

蛋白别名: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; Ly 47; Major group rhinovirus receptor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells

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基因别名: BB2; CD54; ICAM1; P3.58

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UniProt ID: (Human) P05362

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Entrez Gene ID: (Pig) 396750, (Human) 3383

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功能

virus receptor activity receptor activity transmembrane signaling receptor activity integrin binding protein binding

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