Invitrogen

ICAM-1 Monoclonal Antibody (3E2B)

ICAM-1 Antibody in Immunocytochemistry (ICC/IF)

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ICAM-1 Antibody (MA5405) in ICC/IF

Immunofluorescent analysis of CD54 (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD54 monoclonal antibody (Product # MA5405) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLi... View More

ICAM-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ICAM-1 Antibody in Flow Cytometry (Flow)

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产品信息

MA5405

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:10-1:100

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:200

免疫细胞化学 (ICC/IF)

1:10-1:100

流式细胞分析 (Flow)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

中和实验 (Neu)

Assay-dependent

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抑制试剂 (IA)

Assay-dependent

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

3E2B

抗原

Mouse ICAM-1 (CD54)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_223595

产品详细信息

MA5405 targets CD54 in WB, ELISA, FACS, IA, IHC, ICC/IF and Neu applications and shows reactivity with mouse samples.

The MA5405 immunogen is mouse ICAM-1 (CD54).

MA5405 detects CD54 which has a predicted molecular weight of approximately 56 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

靶标信息

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Silencing of lncRNA XIST impairs angiogenesis and exacerbates cerebral vascular injury after ischemic stroke.

Molecular therapy. Nucleic acids

Wang C,Dong J,Sun J,Huang S,Wu F,Zhang X,Pang D,Fu Y,Li L

Published figure using ICAM-1 monoclonal antibody (Product # MA5405) in Western Blot

Fri Dec 03 00:00:00 UTC 2021

Silencing of lncRNA XIST impairs angiogenesis and exacerbates cerebral vascular injury after ischemic stroke.

Molecular therapy. Nucleic acids

Wang C,Dong J,Sun J,Huang S,Wu F,Zhang X,Pang D,Fu Y,Li L

Published figure using ICAM-1 monoclonal antibody (Product # MA5405) in Western Blot

Fri Dec 03 00:00:00 UTC 2021

KLF4 alleviates cerebral vascular injury by ameliorating vascular endothelial inflammation and regulating tight junction protein expression following ischemic stroke.

Journal of neuroinflammation

Zhang X,Wang L,Han Z,Dong J,Pang D,Fu Y,Li L

Published figure using ICAM-1 monoclonal antibody (Product # MA5405) in Western Blot

Tue Apr 07 00:00:00 UTC 2020

KLF4 alleviates cerebral vascular injury by ameliorating vascular endothelial inflammation and regulating tight junction protein expression following ischemic stroke.

Journal of neuroinflammation

Zhang X,Wang L,Han Z,Dong J,Pang D,Fu Y,Li L

Published figure using ICAM-1 monoclonal antibody (Product # MA5405) in Western Blot

Tue Apr 07 00:00:00 UTC 2020

Amelioration of ischemia-reperfusion injury with cyclic peptide blockade of ICAM-1.

American journal of physiology. Heart and circulatory physiology

Merchant SH,Gurule DM,Larson RS

MA5405 was used in blocking/activating experiment to investigate the efficiency of IP25 for the rescue of ischemia induced damage

Tue Apr 01 00:00:00 UTC 2003

Blocking pulmonary ICAM-1 expression ameliorates lung injury in established diet-induced pancreatitis.

Annals of surgery

Lundberg AH,Fukatsu K,Gaber L,Callicutt S,Kotb M,Wilcox H,Kudsk K,Gaber AO

MA5405 was used in blocking/activating experiment to clarify the effect of ICAM-1 expression on the pulmonary injury from pancreatitis

Thu Feb 01 00:00:00 UTC 2001

In vivo microscopy of murine islets of Langerhans: increased adhesion of transferred lymphocytes to islets depends on macrophage-derived cytokines in a model of organ-specific insulitis.

Immunology

Ludwig R,Kretschmer M,Caspar G,Bojunga J,Oldenburg A,Schumm-Draeger P,Stegmüller M,von Minckwitz G,Usadel KH,Kusterer K

MA5405 was used in immunohistochemistry to investigate the mechanism for the lymphocyte adhesion to islets of Langerhans

Wed Sep 01 00:00:00 UTC 1999

生物信息学

蛋白别名: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

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基因别名: CD54; Icam-1; Icam1; Ly-47; MALA-2

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UniProt ID: (Mouse) P13597

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Entrez Gene ID: (Mouse) 15894

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功能

integrin binding protein complex binding

参与通路