Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 5
ICAM-1 Antibody (710278) in ICC/IF
Immunofluorescent analysis of ICAM-1 /CD54 was performed on 90% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ICAM-1 /CD54 Recombinant Rabbit Superclonal™ Antibody (Product # 710278) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG secondary antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temper... View More
产品信息
710278
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
9HCLC
抗原
peptide corresponding to amino acids 504-517 of human Intercellular adhesion molecule-1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532660
产品详细信息
This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells
基因别名: BB2; CD54; ICAM1; P3.58
UniProt ID: (Human) P05362
Entrez Gene ID: (Human) 3383
ovarian follicle development
regulation of leukocyte mediated cytotoxicity
response to amphetamine
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
acute inflammatory response to antigenic stimulus
T cell antigen processing and presentation
positive regulation of cellular extravasation
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell aging
sensory perception of sound
regulation of cell shape
response to ionizing radiation
response to sulfur dioxide
response to organic cyclic compound
membrane to membrane docking
regulation of cell adhesion
extracellular matrix organization
positive regulation of actin filament polymerization
cellular response to nutrient levels
cell adhesion mediated by integrin
response to gonadotropin
response to drug
response to amino acid
positive regulation of GTPase activity
adhesion of symbiont to host
positive regulation of nitric oxide biosynthetic process
response to ethanol
positive regulation of vasoconstriction
response to copper ion
viral entry into host cell
receptor-mediated virion attachment to host cell
positive regulation of peptidyl-tyrosine phosphorylation
regulation of immune response
leukocyte migration
positive regulation of NF-kappaB transcription factor activity
negative regulation of calcium ion transport
interferon-gamma-mediated signaling pathway
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to alkaloid
cellular response to glucose stimulus
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to hypoxia
establishment of Sertoli cell barrier
regulation of ruffle assembly
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of endothelial cell apoptotic process
