Invitrogen

ICAM-1 Monoclonal Antibody (MEM-111), FITC

ICAM-1 Antibody in Flow Cytometry (Flow)

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ICAM-1 Antibody (MHCD5401) in Flow

Clinical interventions in aging 2016 - Figure 2 Flow cytometer analysis of endothelial adhesion molecules, ( A ) VCAM-1 and ( B ) ICAM-1, expression in HUVEC incubated with vehicle CuSO 4 (10 umol/L) + 0.1 mmol/L EDTA (gray bar), Nat Alb (white bars), or Ox Alb (black bars). Notes: HUVEC were treated with different doses (mg/mL) of native and oxidized albumin for 24 hours. MFI are presented as mean +- SD, n=3, in duplicate. * P <0.05 versus vehi... View More

ICAM-1 Antibody in Immunohistochemistry (IHC)

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产品信息

MHCD5401

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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流式细胞分析 (Flow)

Assay-Dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

MEM-111

抗原

Human CD54

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with 4mg/mL BSA

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_10373958

产品详细信息

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

靶标信息

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

High phosphate induces a pro-inflammatory response by vascular smooth muscle cells and modulation by vitamin D derivatives.

Clinical science (London, England : 1979)

Martínez-Moreno JM,Herencia C,de Oca AM,Díaz-Tocados JM,Vergara N,Gómez-Luna MJ,López-Argüello SD,Camargo A,Peralbo-Santaella E,Rodríguez-Ortiz ME,Canalejo A,Rodríguez M,Muñoz-Castañeda JR,Almadén Y

MHCD5401 was used in flow cytometry to test if high phosphate is a pro-inflammatory factor in VSMCs

Sat Jul 01 00:00:00 UTC 2017

Low-dose acetaminophen induces early disruption of cell-cell tight junctions in human hepatic cells and mouse liver.

Scientific reports

Gamal W,Treskes P,Samuel K,Sullivan GJ,Siller R,Srsen V,Morgan K,Bryans A,Kozlowska A,Koulovasilopoulos A,Underwood I,Smith S,Del-Pozo J,Moss S,Thompson AI,Henderson NC,Hayes PC,Plevris JN,Bagnaninchi PO,Nelson LJ

MHCD5401 was used in flow cytometry to show low doses of acetaminophen disrupts the integrity of tight junction and cell-matrix adhesions

Mon Jan 30 00:00:00 UTC 2017

Aging-associated oxidized albumin promotes cellular senescence and endothelial damage.

Clinical interventions in aging

Luna C,Alique M,Navalmoral E,Noci MV,Bohorquez-Magro L,Carracedo J,Ramírez R

Published figure using ICAM-1 monoclonal antibody (Product # MHCD5401) in Flow Cytometry

Wed Sep 28 00:00:00 UTC 2016

TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity.

Frontiers in physiology

Luna C,Carmona A,Alique M,Carracedo J,Ramirez R

Published figure using ICAM-1 monoclonal antibody (Product # MHCD5401) in Flow Cytometry

Wed Jan 06 00:00:00 UTC 2016

Effect of intravenous coadministration of human stroma cell lines on engraftment of long-term repopulating clonal myelodysplastic syndrome cells in immunodeficient mice.

Blood cancer journal

Li X,Marcondes AM,Ragoczy T,Telling A,Deeg HJ

Published figure using ICAM-1 monoclonal antibody (Product # MHCD5401) in Immunofluorescence

Fri Apr 26 00:00:00 UTC 2013

Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.

Journal of virology

Hertel L,Lacaille VG,Strobl H,Mellins ED,Mocarski ES

MHCD5401 was used in flow cytometry to report that CD34(+) progenitor cell-derived Langerhans cells-type DCs exhibit a differentiation state-dependent susceptibility to CMV infection.

Tue Jul 01 00:00:00 UTC 2003

生物信息学

蛋白别名: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells

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基因别名: BB2; CD54; ICAM1; P3.58

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UniProt ID: (Human) P05362

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Entrez Gene ID: (Human) 3383

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功能

virus receptor activity receptor activity transmembrane signaling receptor activity integrin binding protein binding

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