Invitrogen

IDE Monoclonal Antibody (OTI4E5)

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抗体检测数据 (2)
高级验证 (2)

IDE Antibody in Western Blot (WB)

IDE Antibody in Western Blot (WB)

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IDE Antibody (MA5-27528) in WB

Western blot analysis of IDE in HepG2, SVT2, A549, HeLa, COS7 cells using 35 µg per lane. Samples were probed with IDE (Product # MA5-27528) monoclonal antibody at a dilution of 1:500.

IDE Antibody in Western Blot (WB)

IDE Antibody in Western Blot (WB)

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IDE Antibody in Western Blot (WB) — Western blot analysis of IDE in HepG2, SVT2, A549, HeLa, COS7 cells using 35 µg per lane. Samples were probed with IDE (Product # MA5-27528) monoclonal antibody at a dilution of 1:500.

IDE Antibody in Western Blot (WB) — Western blot analysis of IDE in HeLa wild-type and knockout cells using 10 µg per lane. Samples were separated by SDS-PAGE and probed with IDE (Product # MA5-27528) monoclonal antibody and HSP90AB1 antibody with a dilution of 1:200 as a loading control.

IDE Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in IDE knockout (KO) HeLa cell line using Anti-IDE Monoclonal Antibody (OTI4E5) (Product # MA5-27528). {KO}

IDE Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in IDE knockout (KO) HeLa cell line using Anti- (Product # MA5-27528). {KO}

产品信息

MA5-27528

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

-

产品规格

种属反应

Human, Non-human primate, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

OTI4E5

抗原

Human recombinant protein fragment corresponding to amino acids 800-1019 of IDE produced in E.coli

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2723256

靶标信息

This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Abeta-degrading protease; FLJ35968; Insulin degrading enzyme; Insulin protease; Insulin-degrading enzyme; Insulinase; Insulysin

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基因别名: IDE; INSDEGM; INSULYSIN

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UniProt ID: (Human) P14735, (Rat) P35559

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Entrez Gene ID: (Human) 3416, (Rat) 25700

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beta-amyloid binding virus receptor activity glycoprotein binding metalloendopeptidase activity receptor binding protein binding ATP binding zinc ion binding ATPase activity beta-endorphin binding peptide binding protein homodimerization activity ubiquitin binding insulin binding peptidase activity peptide hormone binding metalloprotease

proteolysis insulin receptor signaling pathway determination of adult lifespan bradykinin catabolic process ubiquitin homeostasis protein processing positive regulation of protein oligomerization hormone catabolic process negative regulation of proteolysis viral entry into host cell beta-amyloid metabolic process protein homooligomerization protein homotetramerization protein heterooligomerization proteolysis involved in cellular protein catabolic process insulin metabolic process insulin catabolic process peptide catabolic process cellular protein catabolic process