Invitrogen

IFITM3 Recombinant Rabbit Monoclonal Antibody (JU73-02)

IFITM3 Antibody in Immunocytochemistry (ICC/IF)

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IFITM3 Antibody (MA5-32798) in ICC/IF

Immunofluorescence analysis of Interferon-induced transmembrane protein 3 was performed using 70% confluent log phase SH-SY5Y IFN gamma, 100 units/mL for 24 hrs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IFITM3 Recombinant Rabbit Monoclonal Antibody (JU73-02) (Product # MA5-32798) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit Ig... View More

IFITM3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IFITM3 Antibody in Flow Cytometry (Flow)

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产品信息

MA5-32798

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:1,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JU73-02

抗原

Synthetic peptide within Human Fragilis aa 1-50

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2810074

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

IFITM3 (interferon induced transmembrane protein 3), also known as 1-8U or IP15, is a multi-pass membrane protein that belongs to the IFITM (interferon inducible transmembrane) family of proteins. IFITM proteins are induced by type I and type II interferons and contain multiple interferon (IFN)-stimulated response elements (ISREs) in their promoter regions. IFITM proteins play important roles in many cellular processes and their expression requires the presence of the chromatin remodeling SWI/SNF-like BAF complexes. Cellular processes involving IFITM proteins include cellular anti-proliferative activities and homotypic cell adhesion functions of interferons. In addition, IFITM genes are often upregulated in various cancer cells, suggesting a possible role in carcinogenesis. Localizing to the membrane, IFITM3 is a 133 amino acid protein that is induced by IFN-alpha and IFN-gamma. IFITM3 expression can be regulated by TEF-1, Brg-1 and Sp1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。