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IFN gamma Antibody (500-P32BT-25UG) in WB
Western Blot: To detect Human IFN-gamma by Western Blot analysis IFN gamma Polyclonal Antibody, Biotin (Product # 500-P32BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P32BT-25UG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human IFN-gamma
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930453
产品详细信息
AA Sequence of recombinant protein: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IFN-gamma. Anti-Human IFN-gamma-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human IFN-gamma by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IFN-gamma (500-P32) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IFN-gamma.
Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P32BT-1MG will be provided as 2 x 500 µg
靶标信息
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g
基因别名: IFG; IFI; IFNG
UniProt ID: (Human) P01579
Entrez Gene ID: (Human) 3458
protein import into nucleus, translocation
negative regulation of transcription from RNA polymerase II promoter
neutrophil apoptotic process
regulation of the force of heart contraction
adaptive immune response
CD8-positive, alpha-beta T cell differentiation involved in immune response
apoptotic process
movement of cell or subcellular component
humoral immune response
cell cycle arrest
cell surface receptor signaling pathway
positive regulation of cell proliferation
response to virus
positive regulation of autophagy
positive regulation of gene expression
negative regulation of gene expression
positive regulation of epithelial cell migration
antigen processing and presentation
neutrophil chemotaxis
negative regulation of epithelial cell differentiation
endoplasmic reticulum unfolded protein response
negative regulation of myelination
positive regulation of synaptic transmission, cholinergic
negative regulation of interleukin-17 production
positive regulation of interleukin-12 production
positive regulation of interleukin-23 production
positive regulation of tumor necrosis factor production
positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response
positive regulation of peptidyl-serine phosphorylation of STAT protein
positive regulation of smooth muscle cell apoptotic process
positive regulation of T cell proliferation
response to drug
positive regulation of tyrosine phosphorylation of Stat1 protein
defense response to bacterium
defense response to protozoan
negative regulation of growth of symbiont in host
positive regulation of chemokine biosynthetic process
positive regulation of interleukin-12 biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of interleukin-6 biosynthetic process
positive regulation of nitric oxide biosynthetic process
positive regulation of neuron differentiation
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgG isotypes
negative regulation of smooth muscle cell proliferation
positive regulation of interleukin-1 beta secretion
regulation of insulin secretion
T cell receptor signaling pathway
sensory perception of mechanical stimulus
positive regulation of membrane protein ectodomain proteolysis
defense response to virus
positive regulation of killing of cells of other organism
interferon-gamma-mediated signaling pathway
regulation of interferon-gamma-mediated signaling pathway
positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
positive regulation of fructose 1,6-bisphosphate metabolic process
positive regulation of vitamin D biosynthetic process
positive regulation of calcidiol 1-monooxygenase activity
cellular response to lipopolysaccharide
cellular response to interleukin-18
positive regulation of establishment of protein localization to plasma membrane
extrinsic apoptotic signaling pathway
regulation of neuronal action potential
positive regulation of exosomal secretion
positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
regulation of hepatocyte proliferation
