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IFT25 Antibody (PA5-100040) in ICC/IF
Immunofluorescent analysis of IFT25 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and IFT25 polyclonal antibody (Product # PA5-100040) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-100040
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human HSPB11(Accession Q9Y547), corresponding to amino acid residues C7-V57.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2815570
产品详细信息
Antibody detects endogenous levels of total IFT25.
靶标信息
HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Heat shock protein; Heat shock protein beta-11; heat shock protein family B (small), member 11; Heat shock protein family B member 11; HSP; Hspb11; Hspb11 heat shock protein family B (small), member 11; intraflagellar transport 25 homolog; Intraflagellar transport protein 25 homolog; Placental protein 25; placental protein 25 homolog; PP25
基因别名: 2900042B11Rik; C1orf41; HSPB11; HSPC034; HSPCO34; IFT25; PP25
UniProt ID: (Human) Q9Y547, (Mouse) Q9D6H2
Entrez Gene ID: (Human) 51668, (Mouse) 72938, (Rat) 685284
