Invitrogen

IFT88 Polyclonal Antibody

IFT88 Antibody in Immunocytochemistry (ICC/IF)

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IFT88 Antibody (PA5-18467) in ICC/IF

Immunocytochemistry analysis of IFT88 using IFT88 Polyclonal Antibody (Product # PA5-18467) in paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Product Image Gallery

IFT88 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with IFT88 siRNA and loss of signal was observed in Western Blot using IFT88 Polyclonal Antibody (Product # PA5-18467). {KD}

Product Details

PA5-18467

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

10 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

10 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Published species

Dog, Human, Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (KKRIDEDDFADEE) corresponding to the C-terminus amino acids of IFT88 (aa 804-816).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_10979641

Product Specific Information

This antibody is predicted to react with canine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

Target Information

This gene encodes a member of the tetratrico peptide repeat family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cilia proteins are biomarkers of altered flow in the vasculature.

JCI insight

Gupta A,Thirugnanam K,Thamilarasan M,Mohieldin AM,Zedan HT,Prabhudesai S,Griffin MR,Spearman AD,Pan A,Palecek SP,Yalcin HC,Nauli SM,Rarick KR,Zennadi R,Ramchandran R

PA5-18467 was used in Western Blot to suggest that cilia and ciliary proteins in circulation are detectable under various altered-flow conditions, which could serve as a surrogate biomarker of the damaged endothelium.

Tue Mar 22 00:00:00 UTC 2022

Cilia proteins are biomarkers of altered flow in the vasculature.

JCI insight

Gupta A,Thirugnanam K,Thamilarasan M,Mohieldin AM,Zedan HT,Prabhudesai S,Griffin MR,Spearman AD,Pan A,Palecek SP,Yalcin HC,Nauli SM,Rarick KR,Zennadi R,Ramchandran R

Tue Mar 22 00:00:00 UTC 2022

Ciliogenesis-coupled accumulation of IFT-B proteins in a novel cytoplasmic compartment.

Genes to cells : devoted to molecular & cellular mechanisms

Lamri L,Twan WK,Katoh TA,Botilde Y,Takaoka K,Ikawa Y,Nishimura H,Fukumoto A,Minegishi K,Mizuno K,Hamada H

PA5-18467 was used in Immunocytochemistry to examine the behaviors of the Cluap1 protein, a ciliary protein that belongs to the IFT-B complex and is required for ciliogenesis, in nonciliated and ciliated cells.

Fri Nov 01 00:00:00 UTC 2019

Dicam promotes proliferation and maturation of chondrocyte through Indian hedgehog signaling in primary cilia.

Osteoarthritis and cartilage

Han S,Park HR,Lee EJ,Jang JA,Han MS,Kim GW,Jeong JH,Choi JY,Beier F,Jung YK

PA5-18467 was used in Immunohistochemistry-immunofluorescence to show that, in chondrocytes, Dicam can modulate primary cilia-mediated Indian hedgehog signaling to promote elongation of long bone.

Sun Jul 01 00:00:00 UTC 2018

Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.

Nature communications

Kim H,Xu H,Yao Q,Li W,Huang Q,Outeda P,Cebotaru V,Chiaravalli M,Boletta A,Piontek K,Germino GG,Weinman EJ,Watnick T,Qian F

Published figure using IFT88 polyclonal antibody (Product # PA5-18467) in Western Blot

Tue Nov 18 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: HGNC:20606; intraflagellar transport 88 homolog; Intraflagellar transport protein 88 homolog; MGC26259; polaris homolog; probe hTg737 (polycystic kidney disease, autosomal recessive); Recessive polycystic kidney disease protein Tg737; Recessive polycystic kidney disease protein Tg737 homolog; RP11-172H24.2; testicular tissue protein Li 93; tetratricopeptide repeat domain 10; Tetratricopeptide repeat protein 10; Tg737 protein; TgN(Imorpk)737Rpw; TPR repeat protein 10; transgene insert site 737, insertional mutation, polycystic kidney disease

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Gene Aliases: AW552028; D13S1056E; DAF19; flexo; fxo; hTg737; IFT88; orpk; polaris; TG737; Tg737Rpw; TgN737Rpw; TTC10

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UniProt ID: (Human) Q13099, (Mouse) Q61371

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Entrez Gene ID: (Human) 8100, (Mouse) 21821

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Function(s)

protein binding kinesin binding

Process(es)

cilium morphogenesis regulation of cilium assembly regulation of autophagosome assembly eye development kidney development liver development epithelial cell morphogenesis Notch signaling pathway smoothened signaling pathway sperm axoneme assembly spermatid nucleus elongation determination of left/right symmetry nervous system development brain development heart development protein localization epidermis development regulation of smoothened signaling pathway organ morphogenesis anterior/posterior pattern specification dorsal/ventral pattern formation spinal cord dorsal/ventral patterning telencephalon development cell projection organization lung development pancreas development cytoplasmic microtubule organization response to fluid shear stress nonmotile primary cilium assembly epidermal stem cell homeostasis cilium assembly regulation of odontogenesis of dentin-containing tooth embryonic digit morphogenesis positive regulation of insulin-like growth factor receptor signaling pathway regulation of fat cell differentiation forebrain morphogenesis negative regulation of epithelial cell proliferation cardiac muscle cell differentiation palate development inner ear receptor stereocilium organization limb development regulation of feeding behavior cardiac septum morphogenesis lung vasculature development heart formation neural precursor cell proliferation regulation of protein processing cochlea development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IFT88 Polyclonal Antibody

IFT88 Antibody (PA5-18467) in ICC/IF

Product Details

PA5-18467

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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