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FIGURE: 1 / 2
IGF1R Antibody (H00003480-D01P) in WB
Western Blot analysis of IGF1R expression in transfected 293T cell line (H00003480-T01) by IGF1R MaxPab polyclonal antibody.
Lane 1: IGF1R transfected lysate(154.80 KDa).
Lane 2: Non-transfected lysate.
Lane 1: IGF1R transfected lysate(154.80 KDa).
Lane 2: Non-transfected lysate.
Product Details
H00003480-D01P
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
IGF1R (NP_000866.1, 1 a.a. approximately 1367 a.a) full-length human protein.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: MKSGSGGGSP TSLWGLLFLS AALSLWPTSG EICGPGIDIR NDYQQLKRLE NCTVIEGYLH ILLISKAEDY RSYRFPKLTV ITEYLLLFRV AGLESLGDLF PNLTVIRGWK LFYNYALVIF EMTNLKDIGL YNLRNITRGA IRIEKNADLC YLSTVDWSLI LDAVSNNYIV GNKPPKECGD LCPGTMEEKP MCEKTTINNE YNYRCWTTNR CQKMCPSTCG KRACTENNEC CHPECLGSCS APDNDTACVA CRHYYYAGVC VPACPPNTYR FEGWRCVDRD FCANILSAES SDSEGFVIHD GECMQECPSG FIRNGSQSMY CIPCEGPCPK VCEEEKKTKT IDSVTSAQML QGCTIFKGNL LINIRRGNNI ASELENFMGL IEVVTGYVKI RHSHALVSLS FLKNLRLILG EEQLEGNYSF YVLDNQNLQQ LWDWDHRNLT IKAGKMYFAF NPKLCVSEIY RMEEVTGTKG RQSKGDINTR NNGERASCES DVLHFTSTTT SKNRIIITWH RYRPPDYRDL ISFTVYYKEA PFKNVTEYDG QDACGSNSWN MVDVDLPPNK DVEPGILLHG LKPWTQYAVY VKAVTLTMVE NDHIRGAKSE ILYIRTNASV PSIPLDVLSA SNSSSQLIVK WNPPSLPNGN LSYYIVRWQR QPQDGYLYRH NYCSKDKIPI RKYADGTIDI EEVTENPKTE VCGGEKGPCC ACPKTEAEKQ AEKEEAEYRK VFENFLHNSI FVPRPERKRR DVMQVANTTM SSRSRNTTAA DTYNITDPEE LETEYPFFES RVDNKERTVI SNLRPFTLYR IDIHSCNHEA EKLGCSASNF VFARTMPAEG ADDIPGPVTW EPRPENSIFL KWPEPENPNG LILMYEIKYG SQVEDQRECV SRQEYRKYGG AKLNRLNPGN YTARIQATSL SGNGSWTDPV FFYVQAKTGY ENFIHLIIAL PVAVLLIVGG LVIMLYVFHR KRNNSRLGNG VLYASVNPEY FSAADVYVPD EWEVAREKIT MSRELGQGSF GMVYEGVAKG VVKDEPETRV AIKTVNEAAS MRERIEFLNE ASVMKEFNCH HVVRLLGVVS QGQPTLVIME LMTRGDLKSY LRSLRPEMEN NPVLAPPSLS KMIQMAGEIA DGMAYLNANK FVHRDLAARN CMVAEDFTVK IGDFGMTRDI YETDYYRKGG KGLLPVRWMS PESLKDGVFT TYSDVWSFGV VLWEIATLAE QPYQGLSNEQ VLRFVMEGGL LDKPDNCPDM LFELMRMCWQ YNPKMRPSFL EIISSIKEEM EPGFREVSFY YSEENKLPEP EELDLEPENM ESVPLDPSAS SSSLPLPDRH SGHKAENGPG PGVLVLRASF DERQPYAHMN GGRKNERALP LPQSSTC
Target Information
IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGFR has been implicated in a variety of cancers. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD221; CD221 antigen; IGF 1 receptor; IGF 1R; IGF-I receptor; Insulin like growth factor 1 receptor precursor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2
Gene Aliases: CD221; IGF1R; IGFIR; IGFR; JTK13
UniProt ID: (Human) P08069
Entrez Gene ID: (Human) 3480
protein tyrosine kinase activity
insulin-like growth factor-activated receptor activity
insulin receptor binding
protein binding
insulin-like growth factor binding
ATP binding
insulin-like growth factor I binding
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
transmembrane signal receptor
immune response
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
phosphatidylinositol 3-kinase signaling
positive regulation of cell migration
peptidyl-tyrosine autophosphorylation
negative regulation of apoptotic process
positive regulation of DNA replication
regulation of JNK cascade
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
protein tetramerization
inactivation of MAPKK activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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