Invitrogen

IGF2 Polyclonal Antibody

IGF2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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IGF2 Antibody (PA5-47946) in IHC (F)

Immunohistochemical analysis of IGF2 in immersion fixed frozen sections of mouse embryo (E13-17). Samples were incubated in IGF2 polyclonal antibody (Product # PA5-47946) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).

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IGF2 Antibody — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of IGF-2 is shown by decrease in RFU (relative fluorescent units - measure of proliferation) with increasing concentrations of IGF-2 polyclonal antibody (PA5-47946) {Neu}

产品信息

PA5-47946

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

免疫组化（冰冻） (IHC (F))

5-15 µg/mL

中和实验 (Neu)

0.5-2.5 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived recombinant mouse IGF-II Ala25-Glu91

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2609578

产品详细信息

In direct ELISAs, approximately 50% cross-reactivity with recombinant human IGF-II is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。