Invitrogen

IGF2 Monoclonal Antibody (8H1)

IGF2 Antibody in Immunocytochemistry (ICC/IF)

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IGF2 Antibody (MA5-17096) in ICC/IF

Immunofluorescence analysis of HeLa cells using IGF2 monoclonal antibody (Product # MA5-17096) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

IGF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-17096

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:200-1:1,000

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

中和实验 (Neu)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

8H1

抗原

Purified recombinant fragment of human IGF2 (amino acids: 25-180) expressed in E. Coli.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538567

产品详细信息

MA5-17096 targets IGF2 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples.

The MA5-17096 immunogen is purified recombinant fragment of human IGF2 (amino acids: 25-180) expressed in E. Coli.

MA5-17096 detects IGF2 which has a predicted molecular weight of approximately 20.1kDa.

靶标信息

The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Inhibition of the epigenetically activated miR-483-5p/IGF-2 pathway results in rapid loss of meningioma tumor cell viability.

Journal of neuro-oncology

Uhlmann EJ,Mackel CE,Deforzh E,Rabinovsky R,Brastianos PK,Varma H,Vega RA,Krichevsky AM

MA5-17096 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to discover whether miRNAs dysregulated in meningioma, and explore miRNA-associated pathways amenable for therapeutic interventions.

Wed Mar 01 00:00:00 UTC 2023

Inhibition of the epigenetically activated miR-483-5p/IGF-2 pathway results in rapid loss of meningioma tumor cell viability.

Journal of neuro-oncology

Uhlmann EJ,Mackel CE,Deforzh E,Rabinovsky R,Brastianos PK,Varma H,Vega RA,Krichevsky AM

Wed Mar 01 00:00:00 UTC 2023

Inhibition of the epigenetically activated miR-483-5p/IGF-2 pathway results in rapid loss of meningioma tumor cell viability.

Journal of neuro-oncology

Uhlmann EJ,Mackel CE,Deforzh E,Rabinovsky R,Brastianos PK,Varma H,Vega RA,Krichevsky AM

Wed Mar 01 00:00:00 UTC 2023

Knockdown of circ_0113656 assuages oxidized low-density lipoprotein-induced vascular smooth muscle cell injury through the miR-188-3p/IGF2 pathway.

Open medicine (Warsaw, Poland)

Yang M,Luo J,Zhang S,Huang Q,Cao Q

MA5-17096 was used in Western Blotting to analyze the RNA expression of circ_0113656, microRNA-188-3p (miR-188-3p), and insulin-like growth factor 2 (IGF2) by quantitative real-time polymerase chain reaction.

Tue Jul 18 00:00:00 UTC 2023

Knockdown of circ_0113656 assuages oxidized low-density lipoprotein-induced vascular smooth muscle cell injury through the miR-188-3p/IGF2 pathway.

Open medicine (Warsaw, Poland)

Yang M,Luo J,Zhang S,Huang Q,Cao Q

Tue Jul 18 00:00:00 UTC 2023

Sperm-carried IGF2 downregulated the expression of mitogens produced by Sertoli cells: A paracrine mechanism for regulating spermatogenesis?

Frontiers in endocrinology

Cannarella R,Mancuso F,Arato I,Lilli C,Bellucci C,Gargaro M,Curto R,Aglietti MC,La Vignera S,Condorelli RA,Luca G,Calogero AE

MA5-17096 was used in Western Blot to investigate whether the Insulin-like growth factor 2 protein is expressed in human spermatozoa and, if so, its possible role in the cross-talk between germ and Sertoli cells (SCs) during spermatogenesis.

Thu Dec 22 00:00:00 UTC 2022

PRC2 Heterogeneity Drives Tumor Growth in Medulloblastoma.

Cancer research

Yi J,Kim B,Shi X,Zhan X,Lu QR,Xuan Z,Wu J

MA5-17096 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to report that the histone H3K27 methyltransferase polycomb repressor complex 2 (PRC2) is often heterogeneous within individual SHH medulloblastoma tumors.

Tue Aug 16 00:00:00 UTC 2022

Non-islet Cell Hypoglycemia: Case Series and Review of the Literature.

Frontiers in endocrinology

Garla V,Sonani H,Palabindala V,Gomez-Sanchez C,Subauste J,Lien LF

MA5-17096 was used in Immunohistochemistry to present two cases of NICH with different outcomes.

Mon May 25 00:00:00 UTC 2020

生物信息学

蛋白别名: FLJ22066; FLJ44734; H-IGF-2; IGF; IGF-II; Insulin like growth factor; insulin-like growth factor 2 (somatomedin A); Insulin-like growth factor II; insulin-like growth factor II (IGF-II); Insulin-like growth factor II (somatomedin A); insulin-like growth factor II isoform 2 variant 4 preprotein; insulin-like growth factor type 2; MSA; Multiplication-stimulating activity; Multiplication-stimulating polypeptide; preptin; Somatomedin-A; T3M-11-derived growth factor

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基因别名: AL033362; C11orf43; GRDF; Igf-2; IGF-II; IGF2; IGFII; M6pr; Mpr; Peg2; PP1446; PP9974; RNIGF2

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UniProt ID: (Human) Q9UC68, (Rat) P01346, (Mouse) P09535

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Entrez Gene ID: (Human) 3481, (Rat) 24483, (Mouse) 16002

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功能

insulin receptor binding insulin-like growth factor receptor binding hormone activity protein binding growth factor activity receptor activator activity protein serine/threonine kinase activator activity growth factor

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