Invitrogen

IGFBP3 Polyclonal Antibody, PeproTech®

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IGFBP3 Antibody (500-P230-1MG) in WB

Western Blot: To detect Human IGF-BP3 by Western Blot analysis IGFBP3 Polyclonal Antibody (Product # 500-P230-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-BP3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P230-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human IGF-BP3

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2929940

产品详细信息

AA Sequence of recombinant protein: GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IGF-BP3. Anti-Human IGF-BP3-specific antibody was purified by affinity chromatography employing an immobilized Human IGF-BP3 matrix.

Sandwich ELISA: To detect Human IGF-BP3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IGF-BP3 (500-P230Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IGF-BP3.

Western Blot: To detect Human IGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IGF-BP3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

IGFBP3 is a gene that belongs to the insulin-like growth factor binding protein (IGFBP) family. The gene encodes a protein that contains both an IGFBP domain and a thyroglobulin type-I domain. This protein is known to form a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. The complex circulates in the plasma and prolongs the half-life of IGFs while also altering their interaction with cell surface receptors. Various isoforms of the gene have been identified through alternate transcriptional splice variants. Diseases associated with IGFBP3 include Insulin-Like Growth Factor I and Acid-Labile Subunit Deficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; BP53; Growth hormone dependent binding protein; growth hormone-dependent binding protein; IBP-3; IGF binding protein 3; IGF-binding protein 3; IGFBP; IGFBP-3; Insulin like growth factor binding protein; insulin-like growth factor binding protein 3; Insulin-like growth factor binding protein 3 precursor; Insulin-like growth factor-binding protein 3; tcag7.703

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基因别名: BP-53; IBP3; IGFBP3

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UniProt ID: (Human) P17936

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Entrez Gene ID: (Human) 3486

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功能

fibronectin binding protein binding insulin-like growth factor binding protein tyrosine phosphatase activator activity insulin-like growth factor I binding insulin-like growth factor II binding metal ion binding protease inhibitor

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