Invitrogen

IKAROS Polyclonal Antibody

IKAROS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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IKAROS Antibody (PA5-23728) in IHC (P)

Immunohistochemistry analysis of IKAROS in formalin fixed and paraffin embedded human tonsil tissue. Samples were incubated with IKAROS polyclonal antibody (Product # PA5-23728) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

IKAROS Antibody in Flow Cytometry (Flow)

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产品信息

PA5-23728

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:50-1:100

流式细胞分析 (Flow)

1:10-1:50

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免疫沉淀 (IP)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 415-444 amino acids from the C-terminal region of human IKZF1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2541228

靶标信息

DNA-binding protein IKAROS functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter as well as with the promoters for other genes expressed during early stages of B-cell and T-cell development.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

GFI1 Cooperates with IKZF1/IKAROS to Activate Gene Expression in T-cell Acute Lymphoblastic Leukemia.

Molecular cancer research : MCR

Sun W,Guo J,McClellan D,Poeschla A,Bareyan D,Casey MJ,Cairns BR,Tantin D,Engel ME

PA5-23728 was used in Immunoprecipitation to identify a noncanonical transcriptional control mechanism in T-ALL which supports GFI1-mediated transactivation in partnership with IKAROS and suggest competition between LSD1-containing repressive complexes and others favoring transactivation.

Fri Apr 01 00:00:00 UTC 2022

GFI1 Cooperates with IKZF1/IKAROS to Activate Gene Expression in T-cell Acute Lymphoblastic Leukemia.

Molecular cancer research : MCR

Sun W,Guo J,McClellan D,Poeschla A,Bareyan D,Casey MJ,Cairns BR,Tantin D,Engel ME

Fri Apr 01 00:00:00 UTC 2022

Recapitulation of erythropoiesis in congenital dyserythropoietic anaemia type I (CDA-I) identifies defects in differentiation and nucleolar abnormalities.

Haematologica

Scott C,Downes DJ,Brown JM,Beagrie R,Olijnik AA,Gosden M,Schwessinger R,Fisher CA,Rose A,Ferguson DJP,Johnson E,Hill QA,Okoli S,Renella R,Ryan K,Brand M,Hughes J,Roy NBA,Higgs DR,Babbs C,Buckle VJ

PA5-23728 was used in Flow Cytometry to provide important pointers to the pathways affected in CDA-I which for the first time can now be pursued in the tractable culture system utilised here.

Mon Nov 01 00:00:00 UTC 2021

Evaluation of the Small-molecule BRD4 Degrader CFT-2718 in Small-cell Lung Cancer and Pancreatic Cancer Models.

Molecular cancer therapeutics

Sun D,Nikonova AS,Zhang P,Deneka AY,Fitzgerald ME,Michael RE,Lee L,Lilly AC,Fisher SL,Phillips AJ,Nasveschuk CG,Proia DA,Tu Z,Golemis EA

PA5-23728 was used in Western Blot to suggest that BRD-mediated transcriptional suppression merits further exploration in the setting of SCLC.

Sun Aug 01 00:00:00 UTC 2021

Evaluation of the Small-molecule BRD4 Degrader CFT-2718 in Small-cell Lung Cancer and Pancreatic Cancer Models.

Molecular cancer therapeutics

Sun D,Nikonova AS,Zhang P,Deneka AY,Fitzgerald ME,Michael RE,Lee L,Lilly AC,Fisher SL,Phillips AJ,Nasveschuk CG,Proia DA,Tu Z,Golemis EA

PA5-23728 was used in Western Blot to suggest that BRD-mediated transcriptional suppression merits further exploration in the setting of SCLC.

Sun Aug 01 00:00:00 UTC 2021

生物信息学

蛋白别名: CLL-associated antigen KW-6; DNA-binding protein Ikaros; hIk-1; IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1; protein phosphatase 1, regulatory subunit 92; zinc finger protein, subfamily 1A, 1 (Ikaros)

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基因别名: CVID13; Hs.54452; IK1; IKAROS; IKZF1; LyF-1; LYF1; PPP1R92; PRO0758; ZNFN1A1

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UniProt ID: (Human) Q13422

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Entrez Gene ID: (Human) 10320

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功能

DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription regulatory region DNA binding metal ion binding C2H2 zinc finger transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated cell cycle mesoderm development covalent chromatin modification lymphocyte differentiation erythrocyte differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter