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IKAROS Antibody (14-5780-82) in WB
Western blot was performed using Anti-IKAROS Monoclonal Antibody (2A9-mIkaros), eBioscience™ (Product # 14-5780-82) and a 60kDa band corresponding to IKAROS was observed across samples tested along with an uncharacterized (*) band at ~38 kDa and Mouse IgG at ~25 kDa. Nuclear enriched extracts (40 µg lysate) of Mouse Thymus (Lane 1), Mouse Mammary gland (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iB... View More
产品信息
14-5780-82
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
2A9-mIkaros
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10804525
产品详细信息
Description: This 2A9-mIkaros monoclonal antibody reacts with mouse Ikaros, the 70-kDa founding member of the Ikaros family of C2H2-type zinc finger transcription factors. Ikaros is expressed in hematopoietic progenitor cells, thymocytes, mature T and B cells, monocytes, macrophages, and natural killer cells. This protein has also been shown to be expressed in non-immune cells such as neurons. Studies indicate that Ikaros can interact with other family members, namely Aiolos and Eos. Ikaros is involved in thymocyte development, B cell differentiation, cell cycle control, chromatin remodeling, and tumor suppression. Moreover, Ikaros has been implicated in leukemia and lymphoma. Ikaros has been demonstrated to regulate expression of Tdt, CD8a, and Notch target genes.
At least eight Ikaros isoforms exist due to alternative splicing. The 2A9-mIkaros antibody has been reported to recognize Ikaros isoform VI (Ik-6), which is the longest and most predominant.
Applications Reported: This 2A9-mIkaros antibody has been reported for use in western blotting.
Applications Tested: This 2A9-mIkaros antibody has been tested by western blot of reduced mouse splenocytes. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
DNA-binding protein IKAROS functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter as well as with the promoters for other genes expressed during early stages of B-cell and T-cell development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: hIk-1
基因别名: 5832432G11Rik; hlk-1; Ikaros; LyF-1; mKIAA4227; Zfpn1a1; Znfn1a1
Entrez Gene ID: (Mouse) 22778
