Invitrogen

IL-1 alpha Monoclonal Antibody (ALF-161), eBioscience™

IL-1 alpha Antibody in Immunostaining (IS)

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IL-1 alpha Antibody (14-7011-85) in IS

Mouse splenocytes (500,000 cells per well) were activated with LPS (1 µg/mL) for 48 hrs in mouse IL-1 alpha ELISPOT assay. Control well is medium alone.

IL-1 alpha Antibody in Immunohistochemistry (IHC)

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产品信息

14-7011-85

应用

建议稀释比

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免疫组化 (IHC)

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流式细胞分析 (Flow)

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酶联免疫吸附实验 (ELISA)

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功能检测 (Functional)

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抑制试剂 (IA)

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免疫染色 (IS)

1-4 µg/mL

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

ALF-161

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_468395

产品详细信息

Description: The ALF-161 antibody reacts with mouse interleukin-1alpha (IL-1alpha). Mouse IL-1alpha, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is a 17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1alpha is mostly cell-associated, with 23% amino acid homology with IL-1beta. The immune regulatory role of IL-1alpha is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Applications Reported: The ALF-161 antibody has been reported for use in ELISA capture.

Applications Tested: The ALF-161 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-1 alpha (IL-1 alpha) in combination with the biotin anti-mouse Interleukin-1 alpha (IL-1a) polyclonal (13-7111) antibody for detection and recombinant mouse IL-1a (14-8011) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

PIK3CA variants selectively initiate brain hyperactivity during gliomagenesis.

Nature

Yu K,Lin CJ,Hatcher A,Lozzi B,Kong K,Huang-Hobbs E,Cheng YT,Beechar VB,Zhu W,Zhang Y,Chen F,Mills GB,Mohila CA,Creighton CJ,Noebels JL,Scott KL,Deneen B

14-7011 was used in Immunohistochemistry to develop a high-throughput in vivo screening platform and discover several driver variants of PIK3CA, using a native mouse model of glioblastoma.

Sat Feb 01 00:00:00 UTC 2020

Interleukin-1α Mediates Ozone-Induced Myeloid Differentiation Factor-88-Dependent Epithelial Tissue Injury and Inflammation.

Frontiers in immunology

Michaudel C,Maillet I,Fauconnier L,Quesniaux V,Chung KF,Wiegman C,Peter D,Ryffel B

14-7011 was used in Inhibition experiments to study the role of interleukin 1 associated cytokines in response to ozone exposure.

Mon Jun 24 00:00:00 UTC 2019

Pathogen-Mediated Inhibition of Anorexia Promotes Host Survival and Transmission.

Cell

Rao S,Schieber AMP,O'Connor CP,Leblanc M,Michel D,Ayres JS

14-7011 was used in an ELISA assay to study the mechanism by which Salmonella Typhimurium inhibits anorexia.

Thu Jan 26 00:00:00 UTC 2017

Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment.

Nature communications

Ulland TK,Jain N,Hornick EE,Elliott EI,Clay GM,Sadler JJ,Mills KA,Janowski AM,Volk AP,Wang K,Legge KL,Gakhar L,Bourdi M,Ferguson PJ,Wilson ME,Cassel SL,Sutterwala FS

14-7011 was used in an ELISA assay to demonstrate that C57BL/6J mice have a functional defect in NLRP12, which is required by macrophages for effective neutrophil recruitment to inflammatory sites.

Tue Oct 25 00:00:00 UTC 2016

Ly6C(high) monocytes control cerebral toxoplasmosis.

Journal of immunology (Baltimore, Md. : 1950)

Biswas A,Bruder D,Wolf SA,Jeron A,Mack M,Heimesaat MM,Dunay IR

14-7011 was used in Flow cytometry/Cell sorting to indicate the critical importance of recruited Ly6C monocytes upon cerebral toxoplasmosis.

Wed Apr 01 00:00:00 UTC 2015

The alarmin IL-1α is a master cytokine in acute lung inflammation induced by silica micro- and nanoparticles.

Particle and fibre toxicology

Rabolli V,Badissi AA,Devosse R,Uwambayinema F,Yakoub Y,Palmai-Pallag M,Lebrun A,De Gussem V,Couillin I,Ryffel B,Marbaix E,Lison D,Huaux F

14-7011 was used in Functional assays to assess the implication of alarmins (IL-1α, IL-33 and HMGB1) in the lung response to silica particles.

Sat Dec 13 00:00:00 UTC 2014

Nrf2 is essential for cholesterol crystal-induced inflammasome activation and exacerbation of atherosclerosis.

European journal of immunology

Freigang S,Ampenberger F,Spohn G,Heer S,Shamshiev AT,Kisielow J,Hersberger M,Yamamoto M,Bachmann MF,Kopf M

14-7011 was used in an ELISA assay to identify NF-E2-related 2 as an essential positive regulator of inflammasome activation and IL-1-mediated vascular inflammation.

Fri Jul 01 00:00:00 UTC 2011

Inflammation recapitulates the ontogeny of lymphoid stromal cells.

Journal of immunology (Baltimore, Md. : 1950)

Peduto L,Dulauroy S,Lochner M,Späth GF,Morales MA,Cumano A,Eberl G

14-7011 was used in Functional assays to show that LS cells are both programmed during ontogeny and reinduced during inflammation.

Fri May 01 00:00:00 UTC 2009

Approaches to determine expression of inflammatory cytokines.

Methods in molecular biology (Clifton, N.J.)

Amsen D,de Visser KE,Town T

14-7011 was used in an ELISA assay to describe a protocol for quantification of cytokine mRNA.

Mon May 18 00:00:00 UTC 2009

Developmental maturation of synaptic and extrasynaptic GABAA receptors in mouse thalamic ventrobasal neurones.

The Journal of physiology

Peden DR,Petitjean CM,Herd MB,Durakoglugil MS,Rosahl TW,Wafford K,Homanics GE,Belelli D,Fritschy JM,Lambert JJ

Published figure using IL-1 alpha monoclonal antibody (Product # 14-7011-81) in Immunohistochemistry

Fri Feb 15 00:00:00 UTC 2008

生物信息学

蛋白别名: CTLA8; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1α; ILN; Interleukin; interleukin 17; Interleukin-1 alpha; Interleukin-17A; Interleukin1 alpha; RP23-160G19.8

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基因别名: Il-1a; Il1a

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UniProt ID: (Mouse) P01582

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Entrez Gene ID: (Mouse) 16175

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功能

cytokine activity interleukin-1 receptor binding copper ion binding interleukin superfamily

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