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产品信息
13-7109-81
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
JES3-12G8
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_466920
产品详细信息
Description: The JES3-12G8 antibody reacts with human interleukin-10 (IL-10).
Applications Reported: The JES3-12G8 antibody has been reported for use in the detection of human IL-10 by ELISA.
Applications Tested: The JES3-12G8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-10 (IL-10) in combination with the purified JES3-9D7 (14-7108) antibody for capture and recombinant human IL-10 (14-8109) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor
基因别名: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF
UniProt ID: (Human) P22301
Entrez Gene ID: (Human) 3586
response to molecule of bacterial origin
negative regulation of cytokine secretion involved in immune response
negative regulation of chronic inflammatory response to antigenic stimulus
positive regulation of B cell apoptotic process
inflammatory response
cytoplasmic sequestering of NF-kappaB
cell-cell signaling
aging
regulation of gene expression
response to activity
response to inactivity
hemopoiesis
B cell differentiation
leukocyte chemotaxis
negative regulation of myeloid dendritic cell activation
negative regulation of B cell proliferation
negative regulation of interferon-gamma production
negative regulation of interleukin-1 production
negative regulation of interleukin-12 production
negative regulation of interleukin-18 production
negative regulation of interleukin-6 production
negative regulation of interleukin-8 production
negative regulation of tumor necrosis factor production
receptor biosynthetic process
response to insulin
response to carbon monoxide
cellular response to hepatocyte growth factor stimulus
type 2 immune response
B cell proliferation
negative regulation of T cell proliferation
response to drug
negative regulation of tumor necrosis factor biosynthetic process
defense response to bacterium
defense response to protozoan
negative regulation of apoptotic process
negative regulation of growth of symbiont in host
negative regulation of nitric oxide biosynthetic process
regulation of isotype switching
negative regulation of MHC class II biosynthetic process
positive regulation of MHC class II biosynthetic process
negative regulation of interferon-alpha biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
positive regulation of cytokine secretion
negative regulation of membrane protein ectodomain proteolysis
positive regulation of sequence-specific DNA binding transcription factor activity
response to glucocorticoid
regulation of sensory perception of pain
negative regulation of cytokine activity
branching involved in labyrinthine layer morphogenesis
cellular response to lipopolysaccharide
cellular response to estradiol stimulus
negative regulation of chemokine (C-C motif) ligand 5 production
regulation of complement-dependent cytotoxicity
negative regulation of endothelial cell apoptotic process
