Invitrogen

IL-12 p35 Monoclonal Antibody (SNKY35), eFluor™ 660, eBioscience™

IL-12 p35 Antibody in Flow Cytometry (Flow)

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IL-12 p35 Antibody (50-7359-42) in Flow

Normal human peripheral blood cells stimulated with Human IFN gamma Recombinant Protein (Product # 14-8319-80), LPS, and the Imidazoquinoline, R848, in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03) and stained intracellularly with Anti-Human IL-12/23 p40 PE (Product # 12-7235-42) and Mouse IgG1 K Isotype Control (Product # 50-4714-82) (left) or Anti-Human IL-12 p35 eFluor® 660 (right). Cells in the monocyte gate were used for analysis.

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产品信息

50-7359-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.03 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SNKY35

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11219875

产品详细信息

Description: This SNKY35 monoclonal antibody reacts with human p35, a component of two of the four members of the interleukin (IL)-12 family of cytokines. The IL-12 family members are heterodimeric cytokines composed of an alpha-chain, such as p35, and a beta-chain, such as p40. The alpha-chain proteins are thought to be the limiting factors for secretion of IL-12 family member cytokines, as expression of the alpha-chain is more restricted than that of the beta-chain proteins, and the two subunits may need to be co-expressed in order for cytokine secretion to occur. IL-12 family members are secreted primarily by antigen-presenting cells in response to various TLR agonists and other inflammatory stimuli. The exact types of stimuli present and kinetics of stimulation determine the balance of IL-12 family member production.

The p35 protein was first identified as part of the IL-12 cytokine, which is also known as IL-12p70. Secretion is induced by IFN gamma and agonists of TLR3, 4, or 7 and is inhibited by Th2 cytokines. IL-12 is essential for the differentiation and maintenance of Th1 cells. The other subunit, p40, is expressed in much higher quantities than p35, and may be secreted independently as a monomer or homodimer. Free p40 may act as an antagonist of IL-12 function.

More recently, p35 was also found to dimerize with EBI3 (Epstein-Barr Virus-Induced Gene 3) to form IL-35. The biology of IL-35 is incompletely understood, and it is believed to be unique among the IL-12 family members in its expression and function. It is produced by regulatory T cells, and appears to have immunoregulatory functions. IL-35 has been found to suppress the proliferation and function of effector T cells, and has also been observed to induce a regulatory phenotype on naive CD4+ cells cultured in its presence.

Testing of SNKY35 in the context of IL-35 is currently in process. For additional information, please contact technical support.

Applications Reported: This SNKY35 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This SNKY35 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells. IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.

Cell reports. Medicine

Mirlekar B,Wang Y,Li S,Zhou M,Entwistle S,De Buysscher T,Morrison A,Herrera G,Harris C,Vincent BG,Ting JP,Rashid N,Kim WY,Yeh JJ,Pylayeva-Gupta Y

50-7359-42 was used in Flow Cytometry to argue that B cell effector dysfunction in cancer can be due to an active systemic suppression program that can be targeted to synergize with T cell-directed immunotherapy.

Tue Sep 20 00:00:00 UTC 2022

Single-cell transcriptomic analysis reveals disparate effector differentiation pathways in human T_reg compartment.

Nature communications

Luo Y,Xu C,Wang B,Niu Q,Su X,Bai Y,Zhu S,Zhao C,Sun Y,Wang J,Liu M,Sun X,Song G,Cui H,Chen X,Huang H,Wang H,Han M,Jiang E,Shi L,Feng X

50-7359-42 was used in Flow Cytometry to expand the understanding of Treg cell heterogeneity and differentiation and provide a single-cell atlas for the dissection of Treg complexity in health and disease.

Wed Jun 23 00:00:00 UTC 2021

Prostaglandin E2 inhibits IL-23 and IL-12 production by human monocytes through down-regulation of their common p40 subunit.

Molecular immunology

Kalim KW,Groettrup M

Fri Mar 01 00:00:00 UTC 2013

生物信息学

蛋白别名: CLMF p35; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; il 12; IL-12 subunit p35; IL-12, subunit p35; IL-12A; IL12p35; IL35 subunit; ILN; Interleukin; Interleukin 12; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; Interleukin-12 subunit alpha; Interleukin12; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1

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基因别名: CLMF; IL-12A; IL12A; NFSK; NKSF1; P35

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UniProt ID: (Human) P29459

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Entrez Gene ID: (Human) 3592

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功能

cytokine activity interleukin-12 receptor binding protein binding growth factor activity interleukin-12 beta subunit binding interleukin-27 binding protein heterodimerization activity

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