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IL-12 p40 Antibody (PA5-47197) in Neu
Neutralization of IL-12 p40 in mouse splenocytes. Samples were incubated in IL-12 p40 polyclonal antibody (Product # PA5-47197). Recombinant Rat IL‚12 stimulates proliferation in mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‚12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL‚12 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically0.05-0.25 µg/mL.
产品信息
PA5-47197
产品规格
种属反应
Rat
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Sf 21-derived recombinant rat IL-12 p40: Met23-Ser335; p35: Arg23-Ser215
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2610678
产品详细信息
In direct ELISAs, approximatley 50% cross-reactivity with recombinant mouse IL-12 is observed, 5% cross-reactivity with recombinant human IL-12 is observed, and less than 10% cross-reactivity with recombinant feline IL-12 and recombinant canine IL-12 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.30 EU per 1 µg of the antibody by the LAL method.
靶标信息
IL12B (IL-12 p40) encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kDa cytokine receptor like subunit encoded by this gene, and a 35 kDa subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CLMF p35; il 12; IL-12 subunit p35; IL-12A; IL12 p40; IL12A; ILN; Interleukin; Interleukin 12; interleukin-12 p40; interleukin-12 subunit beta; Interleukin12; NKSF1; RP23-388G23.1
基因别名: Il12
Entrez Gene ID: (Rat) 64546
positive regulation of T cell mediated cytotoxicity
positive regulation of defense response to virus by host
natural killer cell activation involved in immune response
positive regulation of T-helper 1 type immune response
positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
negative regulation of inflammatory response to antigenic stimulus
cell cycle arrest
cell surface receptor signaling pathway
response to UV-B
positive regulation of activation of JAK2 kinase activity
cell migration
cytokine-mediated signaling pathway
sensory perception of pain
natural killer cell activation
negative regulation of interleukin-10 production
negative regulation of interleukin-17 production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-17 production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell activation
positive regulation of natural killer cell proliferation
positive regulation of mononuclear cell proliferation
positive regulation of smooth muscle cell apoptotic process
T-helper cell differentiation
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat4 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
defense response to protozoan
negative regulation of growth of symbiont in host
positive regulation of osteoclast differentiation
positive regulation of cell adhesion
negative regulation of smooth muscle cell proliferation
positive regulation of lymphocyte proliferation
defense response to Gram-negative bacterium
positive regulation of NK T cell activation
positive regulation of NK T cell proliferation
defense response to virus
cellular response to lipopolysaccharide
cellular response to interferon-gamma
