Invitrogen

IL-13 Monoclonal Antibody (5A2), Biotin

产品信息

M130B

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

查看9篇已发表文章 9篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rat / IgG

分类

Monoclonal

类型

Antibody

克隆号

5A2

抗原

Recombinant human IL-14

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS with 4% BSA

内含物

proprietary preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_223557

产品详细信息

M130B targets IL-13 in ELISA, and WB applications and shows reactivity with Human samples.

The M130B immunogen is recombinant human IL-14. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

M130B detects IL-13 which has a predicted molecular weight of approximately 13 kDa.

The M130B IL13 antibody (biotinylated conjugate of clone 5A2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody P130E. Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M130B (clone 5A2) and antibody P130E have successfully been used in combination with recombinant IL13 protein SIL13 in ELISA applications.

靶标信息

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The CD226/CD155 interaction regulates the proinflammatory (Th1/Th17)/anti-inflammatory (Th2) balance in humans.

Journal of immunology (Baltimore, Md. : 1950)

Lozano E,Joller N,Cao Y,Kuchroo VK,Hafler DA

M130B was used in ELISA to study the role of the interaction between CD226 and CD155 in regulating the human T-cell pro-inflammatory/anti-inflammatory balance

Tue Oct 01 00:00:00 UTC 2013

Cellular immune responses in human immunodeficiency virus (HIV)-1-infected children: is immune restoration by highly active anti-retroviral therapy comparable to non-progression?

Clinical and experimental immunology

Hainaut M,Verscheure V,Ducarme M,Schandené L,Levy J,Mascart F

M130B was used in ELISA to study cellular immune responses in HIV-1-infected children

Fri Jul 01 00:00:00 UTC 2011

Fibroblasts stimulated via HLA-II molecules produce prostaglandin E₂ and regulate cytokine production from helper T cells.

Laboratory investigation; a journal of technical methods and pathology

Kato-Kogoe N,Ohyama H,Nishimura F,Meguro M,Yoshizawa S,Okada Y,Nakasho K,Yamanegi K,Yamada N,Hata M,Higashi T,Terada N,Matsushita S

M130B was used in ELISA to study the role of HLA-DQ molecules in Th-cell responses

Wed Dec 01 00:00:00 UTC 2010

Can prenatal malaria exposure produce an immune tolerant phenotype? A prospective birth cohort study in Kenya.

PLoS medicine

Malhotra I,Dent A,Mungai P,Wamachi A,Ouma JH,Narum DL,Muchiri E,Tisch DJ,King CL

M130B was used in ELISA to study the effect of the prenatal malaria exposure on immune tolerance

Wed Jul 01 00:00:00 UTC 2009

Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases.

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver

Marotti B,Rocco A,De Colibus P,Compare D,de Nucci G,Staibano S,Tatangelo F,Romano M,Nardone G

M130B was used in ELISA to study the mucosal production of interleukin 13 in Helicobacter pylori-related gastric diseases

Tue Apr 01 00:00:00 UTC 2008

Modulation of the infant immune responses by the first pertussis vaccine administrations.

Vaccine

Mascart F,Hainaut M,Peltier A,Verscheure V,Levy J,Locht C

M130B was used in ELISA to study the interleukin 13 production in infants with different pertussis-tetanus-diphtheria-poliomyelitis vaccines

Thu Jan 04 00:00:00 UTC 2007

Distinct Th1- and Th2-Type prenatal cytokine responses to Plasmodium falciparum erythrocyte invasion ligands.

Infection and immunity

Malhotra I,Mungai P,Muchiri E,Ouma J,Sharma S,Kazura JW,King CL

M130B was used in ELISA to characterize the prenatal immune responses against Plasmodium falciparum erythrocyte invasion ligands

Wed Jun 01 00:00:00 UTC 2005

Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses.

Journal of immunology (Baltimore, Md. : 1950)

Mascart F,Verscheure V,Malfroot A,Hainaut M,Piérard D,Temerman S,Peltier A,Debrie AS,Levy J,Del Giudice G,Locht C

M130B was used in ELISA to investigate the effect of Bordetella pertussis infection on type 1 T cell responses

Sat Feb 01 00:00:00 UTC 2003

Adaptive immune responses of patients with asthma to the attachment (G) glycoprotein of respiratory synctial virus.

The Journal of infectious diseases

Hancock GE,Scheuer CA,Sierzega R,Pryharski KS,McBride JT,Watelet LF,Tebbey PW,Smith JD

M130B was used in ELISA to study the effect of the attachment (G) glycoprotein from respiratory synctial virus on wheezing

Sat Dec 15 00:00:00 UTC 2001

生物信息学

蛋白别名: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody

查看更多收起

基因别名: IL-13; IL13; NC30; P600

查看更多收起

UniProt ID: (Human) P35225

查看更多收起

Entrez Gene ID: (Human) 3596

查看更多收起

功能

cytokine activity interleukin-13 receptor binding protein binding

参与通路