Invitrogen

IL-15 Polyclonal Antibody, PeproTech®

图: 1 / 3

IL-15 Antibody (500-P15-1MG) in WB

Western Blot: To detect Human IL-15 by Western Blot analysis IL-15 Polyclonal Antibody (Product # 500-P15-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-15 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Product Image Gallery

产品信息

500-P15-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

体外检测 (IV)

查看1篇已发表文章 1篇已发表文章

免疫染色 (IS)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human IL-15

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2929496

产品详细信息

AA Sequence of recombinant protein: MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-15. Anti-Human IL-15-specific antibody was purified by affinity chromatography employing an immobilized Human IL-15 matrix.

Sandwich ELISA: To detect Human IL-15 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-15 (500-P15Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-15.

Western Blot: To detect Human IL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-15 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。