Invitrogen

IL-17A Polyclonal Antibody

IL-17A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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IL-17A Antibody (PA5-79470) in IHC (P)

Immunohistochemistry analysis of IL-17A on paraffin-embedded rat pancreas tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with IL-17A polyclonal antibody (Product# PA5-79470) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Devel... View More

IL-17A Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-79470

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

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酶联免疫吸附实验 (ELISA)

1-5 µg/mL (capture)

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived mouse IL17 recombinant protein (Position: A26-A158).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746586

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Effect of three clinical therapies on cytokines modulation in the hip articular cartilage and bone improvement in rat early osteonecrosis of the femoral head.

Biomedical journal

Hsu SL,Jhan SW,Hsu CC,Wu YN,Wu KLH,Kuo CA,Chiu HW,Cheng JH

Published figure using IL-17A polyclonal antibody (Product # PA5-79470) in Immunohistochemistry

Fri Dec 01 00:00:00 UTC 2023

FOXO1 regulates Th17 cell-mediated hepatocellular carcinoma recurrence after hepatic ischemia-reperfusion injury.

Cell death & disease

Ren H,Chen Y,Zhu Z,Xia J,Liu S,Hu Y,Qin X,Zhang L,Ding Y,Xia S,Wang J

PA5-79470 was used in Immunohistochemistry to indicate that FOXO1-Th17/Treg axis exerts a crucial role in IRI-mediated immunologic derangement and HCC recurrence, which could be a promising target for reducing the HCC recurrence after hepatectomy.

Sat Jun 17 00:00:00 UTC 2023

FOXO1 regulates Th17 cell-mediated hepatocellular carcinoma recurrence after hepatic ischemia-reperfusion injury.

Cell death & disease

Ren H,Chen Y,Zhu Z,Xia J,Liu S,Hu Y,Qin X,Zhang L,Ding Y,Xia S,Wang J

Sat Jun 17 00:00:00 UTC 2023

FOXO1 regulates Th17 cell-mediated hepatocellular carcinoma recurrence after hepatic ischemia-reperfusion injury.

Cell death & disease

Ren H,Chen Y,Zhu Z,Xia J,Liu S,Hu Y,Qin X,Zhang L,Ding Y,Xia S,Wang J

Sat Jun 17 00:00:00 UTC 2023

Osteopontin expression and the effect of anti-VLA-4 mAb treatment in experimental autoimmune encephalomyelitis, a mouse model of multiple sclerosis.

Folia neuropathologica

Pyka-Fościak G,Litwin JA,Lis GJ

PA5-79470 was used in Immunohistochemistry to assess the effect of anti-α4β1 integrin (VLA-4) treatment.

Fri Aug 18 00:00:00 UTC 2023

[¹⁸F]FDG PET-MR characterization of aortitis in the IL1rn^-/- mouse model of giant-cell arteritis.

EJNMMI research

Deshayes S,Baugé C,Dupont PA,Simard C,Rida H,de Boysson H,Manrique A,Aouba A

PA5-79470 was used in Immunohistochemistry to investigate the preclinical model of aortitis in BALB/c IL1rn-/- mice using [18F]fluorodeoxyglucose ([18F]FDG) positron emission tomography-magnetic resonance (PET-MR), gamma counting and immunostaining.

Wed Nov 29 00:00:00 UTC 2023

Extracorporeal Shockwave Therapy Modulates the Expressions of Proinflammatory Cytokines IL33 and IL17A, and Their Receptors ST2 and IL17RA, within the Articular Cartilage in Early Avascular Necrosis of the Femoral Head in a Rat Model.

Mediators of inflammation

Cheng JH,Jhan SW,Hsu CC,Chiu HW,Hsu SL

PA5-79470 was used in Immunohistochemistry to elucidate the healing mechanisms of extracorporeal shockwave therapy in the treatment of avascular necrosis of the femoral head.

Thu Mar 31 00:00:00 UTC 2022

Mediators of inflammation

Cheng JH,Jhan SW,Hsu CC,Chiu HW,Hsu SL

PA5-79470 was used in Immunohistochemistry to elucidate the healing mechanisms of extracorporeal shockwave therapy in the treatment of avascular necrosis of the femoral head.

Thu Mar 31 00:00:00 UTC 2022

Upregulation of Chemokines in the Paraventricular Nucleus of the Hypothalamus in Rats with Stress-Induced Hypertension.

Medical science monitor : international medical journal of experimental and clinical research

Wu Q,Chen Y,Zhang W,Song S,Xu Z,Zhang H,Liu L,Sun J

PA5-79470 was used in Western Blotting to report that the expression of CXCL7 was extremely high in PVN of control rats, and was significantly lower in SIH rats.

Tue Nov 17 00:00:00 UTC 2020

Upregulation of Chemokines in the Paraventricular Nucleus of the Hypothalamus in Rats with Stress-Induced Hypertension.

Medical science monitor : international medical journal of experimental and clinical research

Wu Q,Chen Y,Zhang W,Song S,Xu Z,Zhang H,Liu L,Sun J

PA5-79470 was used in Western Blotting to report that the expression of CXCL7 was extremely high in PVN of control rats, and was significantly lower in SIH rats.

Tue Nov 17 00:00:00 UTC 2020

Long-term PM_2.5 exposure increases the risk of non-small cell lung cancer (NSCLC) progression by enhancing interleukin-17a (IL-17a)-regulated proliferation and metastasis.

Aging

Chao X,Yi L,Lan LL,Wei HY,Wei D

PA5-79470 was used in Immunohistochemistry to explore the role of PM2.5 in the etiology of NSCLC.

Thu Jun 18 00:00:00 UTC 2020

Long-term PM_2.5 exposure increases the risk of non-small cell lung cancer (NSCLC) progression by enhancing interleukin-17a (IL-17a)-regulated proliferation and metastasis.

Aging

Chao X,Yi L,Lan LL,Wei HY,Wei D

PA5-79470 was used in Immunohistochemistry to explore the role of PM2.5 in the etiology of NSCLC.

Thu Jun 18 00:00:00 UTC 2020

Long-term PM_2.5 exposure increases the risk of non-small cell lung cancer (NSCLC) progression by enhancing interleukin-17a (IL-17a)-regulated proliferation and metastasis.

Aging

Chao X,Yi L,Lan LL,Wei HY,Wei D

PA5-79470 was used in Immunohistochemistry to explore the role of PM2.5 in the etiology of NSCLC.

Thu Jun 18 00:00:00 UTC 2020

Neutralization of interleukin-17A alleviates burn-induced intestinal barrier disruption via reducing pro-inflammatory cytokines in a mouse model.

Burns & trauma

Song Y,Li Y,Xiao Y,Hu W,Wang X,Wang P,Zhang X,Yang J,Huang Y,He W,Huang C

Published figure using IL-17A polyclonal antibody (Product # PA5-79470) in Western Blot

Tue Apr 12 00:00:00 UTC 2022

生物信息学

蛋白别名: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; ILN; Interleukin; interleukin 17; Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); Interleukin-17A; Interleukin17A; R-IL-17-A

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基因别名: CTLA-8; CTLA8; IL-17; IL-17A; IL17; IL17A

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UniProt ID: (Human) Q16552, (Mouse) Q62386

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Entrez Gene ID: (Human) 3605, (Mouse) 16171, (Rat) 301289

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功能

cytokine activity cytokine receptor binding protein binding

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