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IL-18 Antibody (PA5-47344) in ICC/IF
Immunocytochemistry analysis of IL-18 in immersion fixed human peripheral blood mononuclear cells (PBMCs). Samples were incubated in IL-18 polyclonal antibody (Product # PA5-47344) using a dilution of 5 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (green). Specific staining was localized to cytoplasm.
产品信息
PA5-47344
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rhesus monkey
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived recombinant rhesus macaque IL-18/IL-1F4 Tyr37-Asp193
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2610680
产品详细信息
This antibody detects rhesus macaque IL-18/IL-1F4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse IL-18, recombinant rat IL-18, and recombinant porcine IL-18 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
靶标信息
The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cytokine; Iboctadekin; IFN-gamma-inducing factor; il 18; IL-1 gamma; IL-18; ILN; Interferon gamma-inducing factor; Interleukin; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; Interleukin-18; interleukin-18 proprotein; Interleukin18; MGC12320
基因别名: IGIF; IL-18; IL-1g; IL18; IL1F4
UniProt ID: (Human) Q14116
Entrez Gene ID: (Rhesus monkey) 574151, (Human) 3606
MAPK cascade
angiogenesis
inflammatory response
immune response
cell-cell signaling
positive regulation of gene expression
natural killer cell activation
regulation of cell adhesion
sleep
lipopolysaccharide-mediated signaling pathway
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-17 production
positive regulation of natural killer cell proliferation
positive regulation of tissue remodeling
chemokine biosynthetic process
T-helper 1 type immune response
type 2 immune response
interleukin-2 biosynthetic process
interferon-gamma biosynthetic process
positive regulation of activated T cell proliferation
interleukin-13 biosynthetic process
granulocyte macrophage colony-stimulating factor biosynthetic process
positive regulation of NF-kappaB import into nucleus
positive regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of myoblast differentiation
positive regulation of inflammatory response
positive regulation of NK T cell proliferation
cellular response to organic cyclic compound
