Invitrogen

IL-2 Monoclonal Antibody (S4B6), Functional Grade, eBioscience™

产品信息

16-7020-85

应用

建议稀释比

已发表文章

中和实验 (Neu)

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功能检测 (Functional)

Assay-Dependent

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体外检测 (IV)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

S4B6

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2573099

产品详细信息

Description: The S4B6 monoclonal antibody reacts with mouse interleukin-2 (IL-2). Mouse IL-2 is a 17 kDa factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK cells, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions, making this cytokine a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.

The S4B6 antibody has been reported to have neutralizing activity. Studies have demonstrated that the S4B6 antibody binds a different epitope of IL-2 than JES6-1A12. In mice, S4B6 renders IL-2 incapable of binding to the high affinity IL-2R but maintains its ability to interact with the low affinity receptor. However, IL-2 bound to JES6-1A12 can still interact with the high affinity IL-2R.

Applications Reported: The S4B6 antibody has been reported for use in functional assays.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

IL-7 induces type 2 cytokine response in lung ILC2s and regulates GATA3 and CD25 expression.

Journal of leukocyte biology

Sheikh A,Lu J,Melese E,Seo JH,Abraham N

16-7020-85 was used in ILC2 stimulation to demonstrate that IL-7 regulates the function of ILC2s during airway viral infection and induces GATA3 and CD25 expression.

Tue Nov 01 00:00:00 UTC 2022

Overproduction of IL-2 by Cbl-b deficient CD4⁺ T cells provides resistance against regulatory T cells.

Oncoimmunology

Han S,Chung DC,St Paul M,Liu ZQ,Garcia-Batres C,Elford AR,Tran CW,Chapatte L,Ohashi PS

16-7020 was used in Functional assays to investigate the role of Cbl-b, an E3 ubiquitin ligase, in establishing resistance to Treg-mediated suppression.

Wed Jul 28 00:00:00 UTC 2021

Overproduction of IL-2 by Cbl-b deficient CD4⁺ T cells provides resistance against regulatory T cells.

Oncoimmunology

Han S,Chung DC,St Paul M,Liu ZQ,Garcia-Batres C,Elford AR,Tran CW,Chapatte L,Ohashi PS

16-7020 was used in Functional assays to investigate the role of Cbl-b, an E3 ubiquitin ligase, in establishing resistance to Treg-mediated suppression.

Wed Jul 28 00:00:00 UTC 2021

The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity.

Nature immunology

Castro W,Chelbi ST,Niogret C,Ramon-Barros C,Welten SPM,Osterheld K,Wang H,Rota G,Morgado L,Vivier E,Raeber ME,Boyman O,Delorenzi M,Barras D,Ho PC,Oxenius A,Guarda G

16-7020-85 was used in In Vivo experiments to show metabolism and homeostasis of natural killer cells can be regulated by transcription factor Rfx7.

Wed Aug 01 00:00:00 UTC 2018

Glucosamine Modulates T Cell Differentiation through Down-regulating N-Linked Glycosylation of CD25.

The Journal of biological chemistry

Chien MW,Lin MH,Huang SH,Fu SH,Hsu CY,Yen BL,Chen JT,Chang DM,Sytwu HK

16-7020 was used in Functional assays to suggest that glucosamine may be an important modulator of T cell differentiation and immune homeostasis.

Fri Dec 04 00:00:00 UTC 2015

Antiapoptotic Mcl-1 is critical for the survival and niche-filling capacity of Foxp3⁺ regulatory T cells.

Nature immunology

Pierson W,Cauwe B,Policheni A,Schlenner SM,Franckaert D,Berges J,Humblet-Baron S,Schönefeldt S,Herold MJ,Hildeman D,Strasser A,Bouillet P,Lu LF,Matthys P,Freitas AA,Luther RJ,Weaver CT,Dooley J,Gray DH,Liston A

16-7020 was used in Neutralization experiments to study the regulation of the Foxp3+ T cell pool by proliferation and apoptosis, and specifically the role of antiapoptoic Mcl-1 in T cell survival.

Sun Sep 01 00:00:00 UTC 2013

生物信息学

蛋白别名: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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基因别名: Il-2; Il2

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UniProt ID: (Mouse) P04351

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Entrez Gene ID: (Mouse) 16183

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功能

cytokine activity interleukin-2 receptor binding growth factor activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding actin or actin-binding cytoskeletal protein non-receptor serine/threonine protein kinase

参与通路