Invitrogen

IL-21 Monoclonal Antibody (mhalx21), PE, eBioscience™

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IL-21 Antibody (12-7213-82) in Flow

Staining of 12-day Th17-polarized BALB/c splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Anti-Mouse IL-21 PE. Cells were treated with Brefeldin A (Product # 00-4506-51) alone (left) or PMA, Ionomycin, and Brefeldin A (right) for 5 hours prior to intracellular staining. Cells in the lymphocyte gate were used for analysis.

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产品信息

12-7213-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看7篇已发表文章 7篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

mhalx21

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1834465

产品详细信息

Description: The monoclonal antibody mhalx21 reacts with mouse interleukin-21 (IL-21). IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by NKT, T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFNgamma for Th1, IL-4 for Th2 and IL-17A for Th17, IL-21 is critical for TFH cell effector function. This cytokine plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells.

In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and RORgammat lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and RORgammat, IL-21 also mediates the downregulation of Foxp3. High levels of IL-21 are present in chemically-induced colitis models. IL-21-deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules.

Preliminary data suggest that clone mhalx21 recognizes a different epitope than anti-mouse IL-21 clone FFA21 (Product # 51-7211).

Applications Reported: This mhalx21 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

This clone has been optimized for use with eBioscience IC Fixation and Permeabilization kit (Product # 88-8823-88).

Applications Tested: This mhalx21 antibody has been tested on Th17-polarized splenocytes restimulated with PMA, Ionomycin and Brefeldin A for 5 hours. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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