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IL-23 p19 Antibody (53-7023-82) in Flow
Mouse bone marrow-derived dendritic cells were unstimulated (left) or stimulated with LPS and R848 and treated with monensin overnight (right). The cells were intracellularly stained with Anti-Mouse IL-12/IL-23 p40 PE (Product # 12-7123-41) and Anti-Mouse IL-23 p19 Alexa Fluor® 488. Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
产品信息
53-7023-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
fc23cpg
抗原
mouse IL-23p19 subunit
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574435
产品详细信息
Description: This fc23cpg monoclonal antibody reacts with the p19 subunit of mouse IL-23, a heterodimeric cytokine made up of two disulfide-linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. IL-23 signals through a heterodimeric receptor expressed on activated T cells, macrophages, and dendritic cells. The receptor shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex. Binding of IL-23 to its receptor activates Jak/STAT pathways similarly to IL-12, but the two cytokines exhibit distinct functions. Unlike IL-12, which induces T-bet and Th1 differentiation, IL-23 signaling results in RORgammat expression and supports CD4^+ T cells with the Th17 phenotype
Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as agonists of the beta-glucan receptor, Dectin-1. Our studies suggest donor, kinetics and type of stimulant can result in variation in IL-23 expression levels. Recent publications suggest the p19 subunit may also exist in the absence of association with p40.
Applications Reported: This fc23cpg antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This fc23cpg antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse bone marrow-derived dendritic cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: il 23; IL 23 A; Il-12b; Il-12p40; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12B; Il12p40; il23; ILN; Interleukin; interleukin 12B; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; MGC79388; p40; RP23-388G23.1
基因别名: IL-23; Il23a; p19
UniProt ID: (Mouse) Q9EQ14
Entrez Gene ID: (Mouse) 83430
positive regulation of T cell mediated cytotoxicity
positive regulation of defense response to virus by host
immune system process
positive regulation of T-helper 1 type immune response
inflammatory response
immune response
positive regulation of activation of JAK2 kinase activity
negative regulation of interleukin-10 production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-17 production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell proliferation
T cell proliferation
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat4 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
positive regulation of memory T cell differentiation
innate immune response
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
tissue remodeling
defense response to Gram-negative bacterium
positive regulation of NK T cell activation
positive regulation of NK T cell proliferation
defense response to virus
positive regulation of neutrophil chemotaxis
positive regulation of T-helper 17 type immune response
positive regulation of T-helper 17 cell lineage commitment
