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IL-3 beta Antibody (500-P177GBT-25UG) in WB
Western Blot: To detect Rat IL-3beta by Western Blot analysis IL-3 beta Polyclonal Antibody, Biotin (Product # 500-P177GBT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P177GBT-25UG
产品规格
种属反应
Rat
宿主/亚型
Goat
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Rat IL-3beta
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2929658
产品详细信息
AA Sequence of recombinant protein: MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Rat IL-3beta. Anti-Rat IL-3beta-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat IL-3beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-3beta (500-P177G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-3beta.
Western Blot: To detect Rat IL-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Rat IL-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P177GBT-1MG will be provided as 2 x 500 µg
靶标信息
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hematopoietic growth factor; IL-3; Interleukin; interleukin-3; mast cell growth factor; MCGF; multipotential colony-stimulating factor; P-cell-stimulating factor
Entrez Gene ID: (Rat) 24495
regulation of cell growth
response to hypoxia
positive regulation of protein phosphorylation
positive regulation of myeloid leukocyte differentiation
regulation of glycolytic process
immune response
positive regulation of cell proliferation
response to hormone
regulation of gene expression
negative regulation of autophagy
cytokine-mediated signaling pathway
hemopoiesis
negative regulation of mast cell apoptotic process
myeloid cell apoptotic process
embryonic hemopoiesis
regulation of protein dephosphorylation
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of apoptotic process
positive regulation of DNA replication
positive regulation of JNK cascade
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of mast cell proliferation
extrinsic apoptotic signaling pathway in absence of ligand
